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MAB1976-AF555 Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate

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MAB1976-AF555
100 µL  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Rb, B, Ca, Ch, H, Mk, PoICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMAB1976-AF555
      DescriptionAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Integrin alpha-V
      • Vitronectin receptor subunit alpha
      • CD51
      • Integrin alpha-V heavy chain
      • Integrin alpha-V light chain
      • Integrin αVβ3
      Background InformationIntegrin alpha-V (CD51, Vitronectin receptor subunit alpha; UniProt P06756) and Integrin beta-3 (CD61, GPIIIa, Platelet membrane glycoprotein IIIa; UniProt P05106) are encoded by the ITGAV (MSK8, VNRA; Gene ID 3685) and ITGB3 (GP3A; Gene ID 3690) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue. Due to its involvement in angiogenesis, integrin alphaVbeta3 is among of the most studied integrin receptors.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin αVβ3 in HeLa cells.
      The unconjugated antibody (Cat. No. MAB1976) is shown to be suitable also for functional assay, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation applications.
      Biological Information
      ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cheresh, D.A., and Spiro R.C. (1987). J. Biol. Chem. 262(36):17703-11).
      CloneLM609
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, D.A., and Spiro R.C. (1987). J Biol Chem. 262(36):17703-11; Cheresh, D.A. (1987). Proc. Natl. Acad. Sci. U.S.A. 84(18):6471-6475). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, P.C., et al. (1994). Science. 264(5158):569-571). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Rabbit
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGAV
      • MSK8
      • VNRA
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~116 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin αVβ3 in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MAB1976-AF555 04055977294729

      Documentation

      Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate 2907161
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate 3792043
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3230943 3230943
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3722057 3722057
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3845817 3845817
      Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate -Q2611359 Q2611359

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