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MAB5412 Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1

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MAB5412
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5412
      ReplacesAB5968
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1
      Alternate Names
      • Indoleamine-pyrrole 2,3-dioxygenase
      Background InformationIndoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
      Product Information
      FormatPurified
      Control
      • IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
      PresentationPurified mouse monoclonal IgG3 in buffer containing 0.02 M phosphate, 0.25 M NaCl, 0.1% NaN3, pH 7.6.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Indoleamine 2, 3-dioxygenase Antibody, clone 10.1 is an antibody against Indoleamine 2 for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody worked on lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended.

      Western blot:
      Recognizes a 42-45kDa band in IFN-gamma treated human HeLa cell lines. Mouse IDO runs slightly smaller in blots than human IDO.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenAmino acids 78-184 of human IDO fused to GST.
      Clone10.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with Indolamine 2,3-dioxygenase (IDO). Note IDO is usually expressed in response to various stimuli. A good positive control is HeLa cells stimulated with IFN-gamma, {http://www.upstate.com/browse/productdetail.q.ProductID.e.05-840#}.
      IsotypeIgG3
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteExpected to react with mouse and rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
      Gene Symbol
      • IDO
      • INDO
      • EC 1.13.11.52
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
      COFACTOR: Binds 1 heme group per subunit.
      SIZE: 403 amino acids; 45326 Da
      SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
      Molecular Weight42-45kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and IFNgamma treated HeLa lysates.

      Western blot:
      1:500 dilution of this lot detected IDO on 10 μg of untreated and IFN gamma treated HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      MAB5412 08436037122750