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05-840 Anti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1

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05-840
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-840
      Replaces04-1056
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1
      Alternate Names
      • Indoleamine 2,3-dioxygenase
      • Indoleamine-pyrrole 2,3-dioxygenase
      • indole 2,3-dioxygenase
      • indoleamine-pyrrole 2,3 dioxygenase
      • IDO1
      Background InformationIndoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
      Product Information
      FormatPurified
      Control
      • Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.
      PresentationPurified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-IDO (Indoleamine 2, 3-Dioxygenase) Antibody, clone 10.1 is an antibody against IDO (Indoleamine 2 for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry: A previous lot was used by an independent laboratory in Phoenix cells transfected with an expression vector.

      Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.
      Clone10.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
      Gene Symbol
      • INDO
      • IDO
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
      COFACTOR: Binds 1 heme group per subunit.
      SIZE: 403 amino acids; 45326 Da
      SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
      Molecular Weight42 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      05-840 04053252729461