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MABT1438-100UL Anti-HYAL1 Antibody, clone 1D10

Anti-HYAL1, clone 1D10, Cat. No. MABT1438, is a mouse monoclonal antibody that detects Hyaluronidase-1 and is tested for use in Immunohistochemistry, Western Blotting, and Western Blotting using knockout lysate.

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MABT1438-100UL
100 µL  
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      Replacement Information
      Description
      Catalogue NumberMABT1438-100UL
      DescriptionAnti-HYAL1 Antibody, clone 1D10
      Alternate Names
      • Hyaluronidase-1
      • EC:3.2.1.35
      • Hyal-1
      • Hyaluronoglucosaminidase-1
      • Lung carcinoma protein 1
      • LuCa-1
      Background InformationHyaluronidase-1 (UniProt: Q12794; also known as EC:3.2.1.35, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1) is encoded by the HYAL1 (also known as LUCA1) gene (Gene ID: 3373) in human. Hyaluronidases (HYAL) catalyze the breakdown of hyaluronic acid at the β1-4 linkage between N-acetyl-β-D-glucosamine and D-glucuronic acid to generate smaller oligosaccharides. HYAL is an acid hydrolase with a pH optimum of ~ 3.8. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that is mainly present in the lysosomal compartment. In murine macrophages, HYAL1 is reportedly trafficked to the lysosomes by a Man-6-P-independent secretion/recapture mechanism mediated by the mannose receptor. Its expression is higher in the liver, kidney, and heart and weak expression is observed in lung, placenta, and skeletal muscle. It has also been detected in plasma and in urine. HYAL-1 is also responsible for the production of angiogenic HA fragments. Its presence in urine and change in expression level in bladder and prostate makes in a marker of bladder and prostate cancers. Significantly higher expression of HYA1 is observed in advanced stages of bladder cancer. Inactivating mutations of HYAL1 gene have been linked to mucopolysaccharidosis IX that is characterized by diminished stature and periarticular soft tissue masses with 40 to 90-fold higher levels of hyaluronic acid than in normal serum. (Ref.: Puissant, E., et al. (2014). Traffic. 15(5); 500-515; Chao, KL., et al. (2007). Biochemistry. 46(23); 6911-6920).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-HYAL1, clone 1D10, Cat. No. MABT1438, is a mouse monoclonal antibody that detects Hyaluronidase-1 and is tested for use in Immunohistochemistry, Western Blotting, and Western Blotting using knockout lysate.
      Key Applications
      • Western Blotting
      • Western Blot Using KO Lysates
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected HYAL1 in Mouse liver tissue lysate.

      Western Blot using KO lysates: A 1:500 dilution from a representative lot detected HYAL1 in lysate from wild-type liver tissue, but not in mouse liver with HYAL1 knockout. (Data courtesy of Dr. Barbara Triggs-Raine, Ph.D., University of Manitoba, Canada).

      Western Blotting Analysis: A representative lot detected HYAL1 in Western Blotting application. (Shuttleworth, T., et al. (2002). J Biol Chem. 277(25):23008-18; Martin, D.C., et al. (2008). Hum Mol Genet. 17(13):1904-15; Hemming, R., et al. (2008). Glycobiology. 18(4):280-9; Puissant, E., et al. (2014). Traffic. 15(5):500-15).

      Immunohistochemistry Applications: A representative lot detected HYAL1 in Immunohistochemistry application. (de Sa, V.K., et al. (2015). Braz J Med Biol Res. 48(11):1039-47).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the first 366 amino acids from human Hyaluronidase-1.
      EpitopeUnknown
      Clone1D10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1D10 is a mouse monoclonal antibody that detects Hyaluronidase-1.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Hamster
      • Human
      Species Reactivity NoteHuman, Hamster, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HYAL1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~52 kDa observed; 48.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse kidney tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Hyaluronidase-1 in Mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABT1438-100UL 04065266876161