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ABN1466 Anti-GLS

Anti-GLS, Cat. No. ABN1466, is a highly specific rabbit polyclonal antibody that targets Glutaminase kidney isoform, mitochondrial, and has been tested in Western Blotting.

Anti-GLS: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Gene Symbol: GLS, GLS1, KIAA0838 UniProt Number: O94925
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ABN1466
100 μg  
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      Replacement Information

      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1466
      DescriptionAnti-GLS
      Alternate Names
      • Glutaminase kidney isoform
      • mitochondrial
      • K-glutaminase
      • L-glutamine amidohydrolase
      Background InformationGlutaminase kidney isoform, mitochondrial (UniProt: O94925; also known as EC:3.5.1.2, GLS, K-glutaminase, L-glutamine amidohydrolase) is encoded by the GLS (also known as GLS1, KIAA0838) gene (Gene ID: 2744) in human. GLS catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. During normal acid-base balance, the kidney extraction of plasma glutamine is low, however, during metabolic acidosis, about one third of the plasma glutamine is extracted and metabolized in a single pass through kidneys. Three isoforms of glutaminase have been described in humans. They are produced via alternate splicing. Isoform 1 and 3 are activated by phosphate, while the isoform 3 lacks catalytic activity. Isoform 1 is highly expressed in brain and kidney, but is not detected in liver. On the other hand, isoform 3 is highly expressed in astrocytoma, ganglioglioma, ependymoma. Lower levels of isoform 3 are reported in placenta, lung, pancreas and kidney, but it is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GLS, Cat. No. ABN1466, is a highly specific rabbit polyclonal antibody that targets Glutaminase kidney isoform, mitochondrial, and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected GLS in 10 µg of human kidney tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects the Glutaminase kidney isoform, mitochondrial, in human. Also detects GLS in the neural cortex. It targets an epitope within 16 amino acids fromt he C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GLS
      • GLS1
      • KIAA0838
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~65 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human neural cortex tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected GLS in 10 µg of human neural cortex tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABN1466 04054839198977