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ABC217 Anti-GLI1 Antibody

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ABC217
100 µL  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC217
      ReplacesMABC678
      DescriptionAnti-GLI1 Antibody
      Alternate Names
      • Zinc finger protein GLI1
      • Glioma-associated oncogene
      • Oncogene GLI
      Background InformationZinc finger protein GLI1 is also called Glioma-associated oncogene and Oncogene GLI. GLI1 is a transcriptional activator that regulates specific genes during normal development. GLI1 also mediates SHH signaling (and therefore cell proliferation and differentiation) and possibly is involved in craniofacial, central nervous system, gastrointestinal tract, and digital development. GLI1 is expressed in the testis, myometrium, fallopian tube, and brain.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GLI1 Antibody is a highly specific rabbit polyclonal antibody, that targets Gli1 & has been tested in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected GL1 in A431, HEK293, HEPG2, and PC3 cells.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GLI1 in human cerebellum and thalamus tissues.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GLI1 in rat testis tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human GLI1.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GLI1
      • GLI
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~150 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected GLI1 in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABC217 04053252920646