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AB16982 Anti-Fas Ligand Antibody

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AB16982
50 µg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB16982
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fas Ligand Antibody
      Alternate Names
      • FasL
      References
      Product Information
      FormatAffinity Purified
      PresentationPeptide affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fas Ligand Antibody detects level of Fas Ligand & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blotting: 1:1,000-1:5,000 Rat, mouse and human FASL have 278, 279, and 281 AA residues, respectively (calculated MW 32kDa). Western blot of the oocyte has detected a specific 31kDa band (Hakuno et al. 1996). A 40 kDa FASL has been detected in mouse T cells (Hahne 1995). An alternate detecting reagent, mFAS-Fc (Suda & Nagata 1994) also detected a cell surface protein of about 40 kDa.

      ELISA: 0.5-1μg/mL

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA 13 AA peptide sequence mapping near the C-terminus of human FAS ligand (Mita et al. 1994). This peptide is predicted to be extracellular. The peptide was synthesized with C-terminal Cysteine, coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityHuman FAS Ligand (FASL). The 13-mer human FASL sequence used for antibody production is 92% (12/13 AA) homologous with rat and mouse FASL. Based upon the sequence homology data, anti-human FASL will likely cross react with the mouse/rat FASL.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
      Gene Symbol
      • FAS
      • CD95
      • FAS1
      • FAIM2
      • OTTHUMP00000059646
      • LFG
      • APT1
      • FASTM
      • NMP35
      • APO-1
      • ALPS1A
      • TNFRSF6
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
      SIZE: 335 amino acids; 37732 Da
      SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
      TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
      DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
      DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
      SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      AB16982 04053252333866

      Documentation

      Anti-Fas Ligand Antibody MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-Fas Ligand Antibody Certificati d'Analisi

      TitoloNumero di lotto
      RABBIT ANTI HUMAN FAS LIGAND - 3561549 3561549
      RABBIT ANTI HUMAN FAS LIGAND -2607885 2607885
      RABBIT ANTI HUMAN FAS LIGAND -2637112 2637112
      RABBIT ANTI HUMAN FAS LIGAND -2689056 2689056
      RABBIT ANTI HUMAN FAS LIGAND -2742740 2742740
      RABBIT ANTI HUMAN FAS LIGAND POLYCLONAL ANTIBODY 3063869
      RABBIT ANTI HUMAN FAS LIGAND POLYCLONAL ANTIBODY - 2036081 2036081
      RABBIT ANTI HUMAN FAS LIGAND POLYCLONAL ANTIBODY - 2060466 2060466

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      Prenatal exposure of mice to diethylstilbestrol disrupts T-cell differentiation by regulating Fas/Fas ligand expression through estrogen receptor element and nuclear factor-κB motifs.
      Singh, NP; Singh, UP; Nagarkatti, PS; Nagarkatti, M
      The Journal of pharmacology and experimental therapeutics  343  351-61  2011

      Mostra il sommario
      22888145 22888145
      Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia.
      Hakuno, N, et al.
      Endocrinology, 137: 1938-48 (1996)  1996

      Mostra il sommario
      8612534 8612534
      Characterization of the non-functional Fas ligand of gld mice.
      Hahne, M, et al.
      Int. Immunol., 7: 1381-6 (1995)  1994

      Mostra il sommario
      7495745 7495745
      Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand.
      Takahashi, T, et al.
      Cell, 76: 969-76 (1994)  1993

      Mostra il sommario
      7511063 7511063
      Role of Fas ligand in apoptosis induced by hepatitis C virus infection.
      Mita, E, et al.
      Biochem. Biophys. Res. Commun., 204: 468-74 (1994)  1993

      Mostra il sommario
      7980502 7980502

      Scheda tecnica

      Titolo
      RABBIT ANTI HUMAN FAS LIGAND POLYCLONAL ANTIBODY

      Prodotti e applicazioni correlate

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies