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MABE2031-25UG Anti-FIH Antibody, clone 162C

Anti-FIH, clone 162C, Cat. No. MABE2031, is a mouse monoclonal antibody that detects Factor inhibiting HIF-1 (FIH) and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MABE2031-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABE2031-25UG
      DescriptionAnti-FIH Antibody, clone 162C
      Alternate Names
      • Hypoxia-inducible factor 1-alpha inhibitor (UniProt: Q9NWT6
      • also known as EC:1.14.11.30
      • Factor inhibiting HIF-1
      • FIH-1
      • Hypoxia-inducible factor asparagine hydroxylase
      Background InformationHypoxia-inducible factor 1-alpha inhibitor (UniProt: Q9NWT6; also known as EC:1.14.11.30, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase) is encoded by the HIF1AN (also known as FIH1) gene (Gene ID: 55662) in human. FIH is a widely expressed, Fe(II)-dependent, homodimeric protein that in the presence of oxygen hydroxylates HIF1 at Asn 803 in the C-terminal transactivation domain. This hydroxylation prevents interaction of HIF1 activation domain with the CH-1 domain of the transcriptional co-activator p300 and reduces the transcriptional activity of HIF1 . Its homodimerization is reported to be essential for its catalytic activity. FIH is mainly cytosolic, but its interaction with NOTCH1 results in its nuclear localization and its interaction with ABPA3 results in its perinuclear localization in macrophages. Silencing of FIH gene by RNA Interference is shown to up-regulate HIF-dependent target gene expression in normoxia. (Ref.: Soilleux, EJ., et al. (2005). Histopathology. 47(6); 602-610; Stolze, IP., et al. (2004). J. Biol. Chem. 279(41); 42719-42725; Hewitson, KS., et al. (2002). J. Biol. Chem. 277(29); 26351-26355)
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FIH, clone 162C, Cat. No. MABE2031, is a mouse monoclonal antibody that detects Factor inhibiting HIF-1 (FIH) and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected FIH in Western Blotting applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).

      Immunohistochemistry Applications: A representative lot detected FIH in Immunohistochemistry applications (Soilleux, E.J., et al. (2005). Histopathology. 47(6): 602-10).

      Immunofluorescence Analysis: A representative lot detected FIH in Immunofluorescence applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).

      Immunocytochemistry Analysis: A representative lot detected FIH in Immunocytochemistry applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length recombinant human Hypoxia-inducible factor 1-alpha inhibitor (FIH) expressed in E. coli.
      EpitopeUnknown
      Clone162C
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 162C is a mouse monoclonal antibody that specifically detects Hypoxia-inducible factor 1-alpha inhibitor (FIH).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIF1AN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~40 kDa observed; 40.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected FIH in MCF-7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABE2031-25UG 04065268364543