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MAB3199Z-C Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free

This Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.

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Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: CDH1, CDHE, UVO UniProt Number: P12830
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MAB3199Z-C
100 µg  
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      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, Inhibition, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3199Z-C
      Replaces04-1103
      DescriptionAnti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free
      Alternate Names
      • Cadherin-1
      • CAM 120/80
      • Epithelial cadherin
      • E-cadherin
      • Uvomorulin
      • CD324
      Background InformationCadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Inhibition
      • Immunofluorescence
      Application NotesFlow Cytometry Analysis: 2 µg from a representative lot detected E-Cadherin in 1X10E6 A431 cells.

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)

      Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).

      Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
      Biological Information
      ImmunogenWhole cells corresponding to human E-Cadherin.
      Clone67A4
      Concentration0.5 mg/mL
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH1
      • CDHE
      • UVO
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~97 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      MAB3199Z-C 04053252970542