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05-414 Anti-CrkL Antibody, clone 5-6

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05-414
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-414
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CrkL Antibody, clone 5-6
      Background InformationCRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
      The antibody does not cross-react with CRKI or CRKII.
      Product Information
      FormatPurified
      Control
      • Western Blot and Immunoprecipitation:
        K562 cell lysate
        Immunocytochemistry:
        Hela cells
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationDetect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenFull length GST fusion protein corresponding to Human CRKL
      EpitopeFull-length
      Clone5-6
      HostMouse
      Specificitythe C-terminal SH3 of Human CRKL
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteReacts with human, mouse and rat. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summaryv-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.
      Gene Symbol
      • CRKL
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46109 # May mediate the transduction of intracellular signals.
      SIZE: 303 amino acids; 33777 Da
      SUBUNIT: Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB.
      SIMILARITY: SwissProt: P46109 ## Contains 1 SH2 domain. & Contains 2 SH3 domains.
      Molecular Weight39kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on K562 cell lysate.

      Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      05-414 04053252370212