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ABT123 Anti-Collagen Type I Antibody

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ABT123
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWB, IH(P), ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT123
      ReplacesAB755P
      DescriptionAnti-Collagen Type I Antibody
      Alternate Names
      • Collagen alpha-1(I) chain
      • Alpha-1 type I collagen
      Background InformationCollagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
      Product Information
      FormatAffinity Purified
      Control
      • Rat skin tissue
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Collagen Type I using this Anti-Collagen Type I Antibody validated for use in Western Blotting, IHC(P), ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • ELISA
      Application NotesSpecificity ELISA Analysis: A representative lot detected mouse and rat collagen but not human collagen in ELISA.

      Western Blot Analysis: A 1:2,000 dilution from a representative lot detected Collagen Type 1 on 10 µg of rat skin tissue lysate.
      Biological Information
      ImmunogenRat tail collagen
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COL1A1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight130 kDa calculated
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat skin tissue.

      Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Collagen Type 1 in rat skin tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Numero di catalogo GTIN
      ABT123 04053252647918