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AB756P Anti-Collagen Antibody, Type IV

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AB756P
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, IF, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB756P
      ReplacesAB756
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Collagen Antibody, Type IV
      OverviewCollagen Type IV extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III and mouse Fibronectin and Laminin.
      Background InformationCollagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
      Product Information
      FormatPurified
      Control
      • Positive Control: Kidney, muscle, tendon spleen tissue, mouse liver. Negative Control: Neurons/glia.
      PresentationThe purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Collagen Antibody, Type IV is validated for use in ELISA, IF, IH(P) for the detection of Collagen.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Applications Not Recommended
      • Western Blotting
      Application NotesELISA:
      A previous lot of this antibody was used in ELISA at > 1:200 (OD > 500).

      Immunofluorescence:
      A previous lot of this antibody was used in immunofluorescence.

      Immunohistochemistry:
      1:80 dilution for immunofluorescent staining of fresh frozen mouse skin and liver tissues. Acetone or methyl-carnoy fixed paraffin-embedded tissue (mouse skin and liver) is also reactive.

      Not recommended for Western blots.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenCollagen Type IV extracted and purified from mouse tumor tissues.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III, mouse Fibronectin, and mouse laminin.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse Collagen, Type IV: 100 (%’s at a 1:5000 RIA dilution) Mouse Collagen, Types I, II, III: <0.1 Human Collagen, Types IV, V: <0.1 Mouse Fibronectin: <0.1 Mouse Laminin: <0.1 Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
      SUBUNIT: There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane.
      TISSUE: Brain, Eye, Heart, and Placenta
      Molecular Weight139 kDa (unprocessed precursor)
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Numero di catalogo GTIN
      AB756P 04053252272660