Millipore Sigma Vibrant Logo

217605 Anti-CD54 Mouse mAb (15.2)

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Anti-CD54 Mouse mAb (15.2): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: Anti-ICAM-1, Domain 1

View Products on Sigmaaldrich.com
217605
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
217605-100UG
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 100 μg
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      Description
      OverviewRecognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
      Catalogue Number217605
      Brand Family Calbiochem®
      SynonymsAnti-ICAM-1, Domain 1
      References
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Positive controlRaji cells
      Preservative0.5 mg/ml gentamicin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (1-10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman monocytes
      ImmunogenHuman
      Clone15.2
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      217605-100UG 04055977202892

      Documentation

      Anti-CD54 Mouse mAb (15.2) MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-CD54 Mouse mAb (15.2) Certificati d'Analisi

      TitoloNumero di lotto
      217605

      Riferimenti bibliografici

      Panoramica delle referenze
      Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
      Scheda tecnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-ICAM-1, Domain 1
      ApplicationFlow Cytometry (1-10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
      BackgroundHuman CD54 (Intercellular Adhesion Molecule-1, or ICAM-1) is a ~90 kDa lymphocyte/monocyte cell surface glycoprotein that mediates cell-cell adhesion by interacting with the integrins LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18).
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman monocytes
      Clone15.2
      IsotypeIgG₁
      Specieshuman
      Positive controlRaji cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Purity≥95% by SDS-PAGE
      Preservative0.5 mg/ml gentamicin sulfate
      CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.