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MABC1823-25UG Anti-CD271/p75NTR Antibody, clone G4B1

Anti-CD271/p75NTR, clone G4B1, Cat. No. MABC1823, is a mouse monoclonal antibody that detects CD271 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABC1823-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABC1823-25UG
      DescriptionAnti-CD271/p75NTR Antibody, clone G4B1
      Alternate Names
      • Tumor necrosis factor receptor superfamily member 16
      • Gp80-LNGFR
      • Low affinity neurotrophin receptor p75NTR
      • Low-affinity nerve growth factor receptor
      • p75 ICD
      • CD271
      Background InformationTumor necrosis factor receptor superfamily member 16 (UniProt: P08138; also known as Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, CD271) is encoded by the NGFR (also known as TNFRSF16) gene (Gene ID: 4804) in human. CD271 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 29-250), a transmembrane domain (aa 251-272), and a cytoplasmic domain (aa 273-427). It serves as a low affinity receptor that can bind to NGF, BDNF, NTF3, and NTF4. It forms a heterodimeric receptor with sortilin related VPS10 domain containing receptor 2 (SorCS2) that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, but has much lower affinity for mature NGF and BDNF. CD271 plays an important role in differentiation and survival of specific neuronal populations during development. It can mediate cell survival as well as cell death of neural cells. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 344-421, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type. Clone G4B1 is shown to bind to the fourth cysteine-rich domain (CRD4) in the extracellular domain and is specifically detects glycosylated CD271. It recognizes CD271 expressed in esophageal cancer, but not in normal esophageal basal cells. It also detects the fetal esophageal epithelium and Barrett's esophagus that possess stem cell-like characteristics. (Ref.: Fujii, K., et al. (2022). Cancer Sci. 113(8); 2878-2887; Glerup, S., et al. (2014). Neuron. 82(5); 1074-1087; Baeza-Raja , B., et al. (2013). J Neurosci. 33(25); 10221-10234).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD271/p75NTR, clone G4B1, Cat. No. MABC1823, is a mouse monoclonal antibody that detects CD271 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected CD271 in Immunocytochemistry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

      Flow Cytometry Analysis: A representative lot detected CD271 in Flow Cytometry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

      Immunohistochemistry Applications: A representative lot detected CD271/p75NTR in Immunohistochemistry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

      Western Blotting Analysis: A representative lot detected CD271 in Western Blotting applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

      ELISA Analysis: A representative lot detected CD271 in ELISA applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA recombinant fragment corresponding to the extracellular domain of human CD271/p75NTR.
      EpitopeExtracellular domain
      CloneG4B1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone G4B1 is a mouse monoclonal antibody that detects CD271 (p75ICD). It targets an epitope within the fourth cysteine-rich domain in the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NGFR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight45.18 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HPCM2 cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected CD271/p75NTR in one million HPCM2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABC1823-25UG 04065270321589

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