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MABC34-25UG Anti-Beclin-1 Antibody, clone 9A1.1 (mouse monoclonal)

Anti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry.

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Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BECN1, GT197 UniProt Number: Q14457
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MABC34-25UG
25 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC34-25UG
      DescriptionAnti-Beclin-1 Antibody, clone 9A1.1 (mouse monoclonal)
      Alternate Names
      • Beclin-1
      • Coiled-coil myosin-like BCL2-interacting protein
      • Protein GT197
      Background InformationThe Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
      References
      Product Information
      FormatPurified
      Control
      • Raji cell lysate
      PresentationPurified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in human colorectal adenocarcinoma tissue.

      Flow Cytometry Analysis: 1 µg from a representative lot detected Beclin-1 in HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Beclin-1.
      Clone9A1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BECN1
      • GT197
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~54 kDa kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Raji cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Beclin-1 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABC34-25UG 04054839350429