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MABC1598-25UL Anti-BAK Antibody, clone 7D10

Anti-BAK, clone 7D10, Cat. No. MABC1598, is a rat monoclonal antibody that detects Apoptosis regulator BAK and has been tested for use in Immunoprecipitation, Function Analysis, and Western Blotting.

MABC1598-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberMABC1598-25UL
      DescriptionAnti-BAK Antibody, clone 7D10
      Alternate Names
      • Bcl-2 homologous antagonist/killer
      • Apoptosis regulator BAK
      • Bcl-2-like protein 7
      • Bcl2-L-7
      Background InformationBcl-2 homologous antagonist/killer (UniProt: Q16611; also known as Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7) is encoded by the BAK1 (also known as BAK, BCL2L7, CDN1) gene (Gene ID:578) in human. BAK is a single-pass membrane protein of the Bcl-2 family that is expressed in a wide variety of tissues, with highest levels displayed in heart and skeletal muscle. BAK plays a significant role in the mitochondrial apoptotic process. In non-apoptotic cells, BAK constitutively resides in the mitochondrial outer membrane, but upon arrival of cell death signals, it promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM and releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9. BAK contains three BH motifs (BH1: aa 117-136; BH2: aa 169-184; and BH3: aa 74-88) and intact BH3 motif is required by BIK, BID, BAD, and BAX for their pro-apoptotic activity and their interaction with anti-apoptotic members of the Bcl-2 family. This antibody (clone 7D10) binds close to the alpha1 region in non-activated BAK to induce conformational change, oligomerization, and cytochrome c release. Its binding to BAK is shown to directly impact alpha1, but not alpha2 region. A short sequence (aa 51-55) is shown to be essential for binding of this antibody. (Ref.: Iyer, S., et al. (2016). Nature Commun. 7; 11734).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-BAK, clone 7D10, Cat. No. MABC1598, is a rat monoclonal antibody that detects Apoptosis regulator BAK and has been tested for use in Immunoprecipitation, Function Analysis, and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Function Assay
      • Western Blotting
      Application NotesInduces Function Analysis: A representative lot induced conformational change and oligomerization of BAK and caused cytochrome c release. (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated BAK in Immunoprecipitation applications (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

      Western Blotting Analysis: A representative lot detected BAK in Western Blotting applications (Dewson, G., et. al. (2009). Mol Cell. 36(4):696-703; Kotschy, A., et. al. (2016). Nature. 538(7626):477-482; Alsop, A.E., et. al. (2015). Nat Commun. 6:6841).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 186 amino acids of human BAK.
      Clone7D10
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 7D10 is a rat monoclonal antibody that detects BAK protein in human and murine species. It binds close to alpha1 region in non-activated BAK.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BAK1
      • BAK
      • BCL2L7
      • CDN1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~24 kDa observed; 23.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in wild type and Bax/Bak double knockout HeLa cell lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected BAK in Wild type HeLa cell lysate, but not in Bax/Bak double knockout HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABC1598-25UL 04054839646744