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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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Anti-Aquaporin 2 (254-271), rabbit polyclonal, recognizes the ~40 and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide. It is validated for WB, IHC, and IP.
More>>Anti-Aquaporin 2 (254-271), rabbit polyclonal, recognizes the ~40 and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide. It is validated for WB, IHC, and IP. Less<<
Anti-Aquaporin 2 (254-271) Rabbit pAb: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
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Description
Overview
Recognizes the ~40 kDa and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide (AQP2254-271 immunogen peptide).
Catalogue Number
178612
Brand Family
Calbiochem®
Synonyms
Anti-AQP2, Anti-AQP-CD, Anti-WCH-CD
Application Data
Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence. Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
References
References
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Fushimi, K., et al. 1993. Nature 361, 549. Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA90, 11663.
Product Information
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
Immunoprecipitation
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
Immunohistochemistry
Fushimi, K., et al. 1993. Nature 361, 549.
Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA90, 11663.
Immunoblotting (1:200-1:600) Immunohistochemistry (see application reference) Immunoprecipitation (see application reference)
Application Comments
Immunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogen
a synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
Immunogen
Mouse/Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Please see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Fushimi, K., et al. 1993. Nature 361, 549. Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA90, 11663.
Scheda tecnica
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-February-2008 JSW
Synonyms
Anti-AQP2, Anti-AQP-CD, Anti-WCH-CD
Application
Immunoblotting (1:200-1:600) Immunohistochemistry (see application reference) Immunoprecipitation (see application reference)
Application Data
Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence. Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
Description
Protein A purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-2 proteins. Supplied with a control peptide (AQP2254-271 immunogen peptide).
Background
AQP2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. Defects in AQP2 are a cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).
Host
Rabbit
Immunogen species
Mouse/Rat
Immunogen
a synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
Isotype
IgG
Species
human, mouse, rat
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Immunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Please see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
Toxicity
Irritant
References
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Fushimi, K., et al. 1993. Nature 361, 549. Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA90, 11663.
Application references
Immunoprecipitation
Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
Immunohistochemistry
Fushimi, K., et al. 1993. Nature 361, 549.
Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA90, 11663.
