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ABF1052-25UL Anti-Adenovirus 5 L4-33K

Anti-Adenovirus 5 L4-33K, Cat. No. ABF1052, is a rabbit polyclonal antibody that detects Adenovirus 5 L4-33K and is tested for use in Western Blotting and Immunofluorescence.

ABF1052-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberABF1052-25UL
      DescriptionAnti-Adenovirus 5 L4-33K
      Alternate Names
      • Protein 33K
      • L4-33K
      • Splicing factor 33K
      • Terminase small subunit
      Background InformationProtein 33K (UniProt: P24940; also known as L4-33K, Splicing factor 33K, Terminase, small subunit) is encoded by the L4 gene (Gene ID: 2653005) in human Adenovirus 5. Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. L4-33K is a homooligomeric protein that serves as a virus-encoded alternative RNA splicing factor. It is expressed in the late phase of viral replicative cycle. It activates splicing of viral late gene transcripts that contain weak 3 splice sites. It contains a short arginine-serine repeat region (aa 178-198) that is essential for its splicing enhancer function. L4-33K also plays a role in adenovirus assembly and in adenovirus morphogenesis by promoting the packaging of the viral genome into the empty capsid. It is essential for the temporal activation of major late pre-mRNA splicing at late stages of infection and it induces the splicing and expression of the late capsid vertex protein. It is suggested to function as the small terminase that is part of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. It is not detected in mature virions. The L4-33K protein may be phosphorylated by DNA-dependent protein kinase (DNA-PK) and protein kinase A (PKA). Its phosphorylation by DNA-PK is reported to have an inhibitory action in L4-33K-mediated alternative RNA splicing whereas the phosphorylation by PKA stimulates L4-33K-mediated alternative RNA splicing. Two isoforms of L4-33K have been described that are produced by alternative splicing. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524; Wu, K., et al. (2013). J. Virol. 87(12); 6739-6747).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antisera without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Adenovirus 5 L4-33K, Cat. No. ABF1052, is a rabbit polyclonal antibody that detects Adenovirus 5 L4-33K and is tested for use in Western Blotting and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected Adenovirus L4-33K in Western Blotting application. (Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

      Immunofluorescence Analysis: A representative lot detected Adenovirus L4-33K in Immunofluorescence application. (Charman, M., et al. (2023). Nature. 616(7956):332-338).

      Western Blotting Analysis: A 1:2,500 dilution from a representative lot detected Adenovirus 5 L4-33K in HeLa cells infected with Human adenovirus type 5. (Courtesy of Dr. Patrick Hearing, Stony Brook University).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged, recombinant fragment corresponding to 124 amino acids from the C-terminal half of human adenovirus 5.
      EpitopeUnknown
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Protein 33K of Human Adenovirus 5.
      IsotypeIgG
      Species Reactivity
      • Virus
      Species Reactivity NoteAdenovirus 5.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • L4
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~35 kDa observed; 25.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells infected Human adenovirus type 5.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protein 33K of Adenovirus 5 in lysate from HeLa cells infected with human adenovirus type 5, but not in lysate from uninfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABF1052-25UL 04065271174979