Millipore Sigma Vibrant Logo

MAB4418 Anti-Achaete Scute homolog 2 Antibody, clone 7E2

Anti-Achaete Scute homolog 2 Antibody, clone 7E2 detects level of Achaete Scute homolog 2 & has been published & validated for use in WB, IC, ChIP.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Stem Cell Research Research Sub-Category: Developmental Signaling Gene Symbol: ASCL2, ASH2, HASH2, MASH2, Mash2 UniProt Number: Q99929
View Products on Sigmaaldrich.com
MAB4418
100 µg  
Ricerca del prezzo in corso...
Non è stato possibile trovare il prezzo
La quantità minima deve essere un multiplo di
Maximum Quantity is
Al termine dell'ordine Maggiori informazioni
Lei ha salvato ()
 
—
Richiedi il prezzo
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      Offerte speciali

       

      Contatti il Servizio Clienti

      Panoramica

      Replacement Information

      Offerte speciali

      100% Performance Guaranteed

      Tabella delle specifiche principali

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4418
      DescriptionAnti-Achaete Scute homolog 2 Antibody, clone 7E2
      Alternate Names
      • achaete-scute complex (Drosophila) homolog-like 2
      • achaete-scute complex homolog 2 (Drosophila)
      • achaete-scute complex homolog-like 2
      • achaete-scute complex-like 2
      • achaete-scute complex-like 2 (Drosophila)
      • mammalian achaete/scute homologue 2
      Background InformationAchaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).
      References
      Product Information
      FormatPurified
      Control
      • Human placenta tissue lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Achaete Scute homolog 2 Antibody, clone 7E2 detects level of Achaete Scute homolog 2 & has been published & validated for use in WB, IC, ChIP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemmistry Analysis: A previous lot was used by an independent laboratory in IC (Van de Flier, L.G., et al., 2009)

      Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChIP (Hatzis, P., et al., 2008).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human Achaete Scute homolog 2 near the C-terminus.
      EpitopeC-terminus
      Clone7E2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Achaete Scute homolog 2 near the C-terminus.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. [provided by RefSeq].
      Gene Symbol
      • ASCL2
      • ASH2
      • HASH2
      • MASH2
      • Mash2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.

      SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed specifically in the extravillous trophoblasts of the developing placenta.

      SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight ~20 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human placenta tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 in 10 µg of human placenta tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MAB4418 04053252577031

      Documentation

      Anti-Achaete Scute homolog 2 Antibody, clone 7E2 MSDS

      Titolo

      Scheda di sicurezza (MSDS) 

      Anti-Achaete Scute homolog 2 Antibody, clone 7E2 Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Achaete Scute homolog 2, clone 7E2 - 2455743 2455743
      Anti-Achaete Scute homolog 2, -2567025 2567025
      Anti-Achaete Scute homolog 2, -2688645 2688645
      Anti-Achaete Scute homolog 2, -2716344 2716344
      Anti-Achaete Scute homolog 2, -2762326 2762326
      Anti-Achaete Scute homolog 2, -2808206 2808206
      Anti-Achaete Scute homolog 2, clone 7E2 - 2066321 2066321
      Anti-Achaete Scute homolog 2, clone 7E2 - 2324661 2324661
      Anti-Achaete Scute homolog 2, clone 7E2 - 2495650 2495650
      Anti-Achaete Scute homolog 2, clone 7E2 - 3126655 3126655
      Show more

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      Transcription factor achaete scute-like 2 controls intestinal stem cell fate.
      van der Flier, LG; van Gijn, ME; Hatzis, P; Kujala, P; Haegebarth, A; Stange, DE; Begthel, H; van den Born, M; Guryev, V; Oving, I; van Es, JH; Barker, N; Peters, PJ; van de Wetering, M; Clevers, H
      Cell  136  903-12  2009

      Mostra il sommario       		19269367 19269367

      Scheda tecnica

      Titolo
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation