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MABS2304-100UG Anti-ASGPR sH2a Antibody, clone B9

This mouse monoclonal Anti-ASGPR sH2a, clone B9, Cat. No. MABS2304 is tested for use in ELISA and Immunoprecipitation, for the detection of ASGPR sH2a.

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MABS2304-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABS2304-100UG
      DescriptionAnti-ASGPR sH2a Antibody, clone B9
      Alternate Names
      • Asialoglycoprotein receptor 2
      • ASGP-R 2
      • C-type lectin domain family 4 member H2
      • Hepatic lectin H2
      • HL-2
      Background InformationAsialoglycoprotein receptor 2 (UniProt: P07307; also known as ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2) is encoded by the ASGR2 (also known as CLEC4H2) gene (Gene ID: 433) in human. ASGPR is a type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-58), a transmembrane domain (aa 59-79), and an extracellular domain (aa 80-311). Its C-type lectin domain is localized in amino acids 176-302. ASGPR is expressed exclusively in hepatic parenchymal cells where it serves in clearance of asialoglycoproteins from the plasma. It mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated, and the receptor is returned to the cell membrane surface. A soluble form of ASGPR (~ 35 kDa) has also been described and is termed as sH2a that is formed by cleavage in the endoplasmic reticulum of its precursor. Levels of ASGPR sH2a are significantly reduced under conditions of liver cirrhosis and fibrosis. Clone B9 specifically detects the secreted sH2a form of ASGPR in human plasma and in NIH3T3 cells transfected with human ASGPR sH2a. (Ref.: Shenkman, M., et al. (2013). J. Biol. Chem. 288(4); 2167-2178; Benyair, R., et al. (2011). World J. Gastroenterol. 17(48); 5305-5309).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-ASGPR sH2a, clone B9, Cat. No. MABS2304 is tested for use in ELISA and Immunoprecipitation, for the detection of ASGPR sH2a.
      Key Applications
      • ELISA
      • Immunoprecipitation
      Application NotesTested applications

      Immunoprecipitation Analysis: A representative lot immunoprecipitated ASGPR sH2a in Immunoprecipitation applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9; Shenkman, M., et al. (2013). J Biol Chem. 288(4):2167-78).

      ELISA Analysis: A representative lot detected ASGPR sH2a in ELISA applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Asialoglycoprotein receptor 2.
      EpitopeC-terminal
      CloneB9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone B9 is a mouse monoclonal antibody that detects Asialoglycoprotein receptor 2 (ASGPR2). It targets an epitope within 12 amino acids from the C-terminal region.
      IsotypeIgG3λ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ASGR2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight35.09 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with recombinant human ASGPR protein.

      ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant human ASGPR sH2a protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      MABS2304-100UG 04065269049197