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ABS2210-25UL Anti-ANO6/TMEM16F

Anti-ANO6/TMEM16F, Cat. No. ABS2210, is a rabbit polyclonal antibody that detects Anoctamin-6 and is tested for use in Western Blotting.

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ABS2210-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberABS2210-25UL
      DescriptionAnti-ANO6/TMEM16F
      Alternate Names
      • Anoctamin-6
      • Small-conductance calcium-activated nonselective cation channel
      • SCAN channel
      • Transmembrane protein 16F
      Background InformationAnoctamin-6 (UniProt: Q6P9J9; also known as Small-conductance calcium-activated nonselective cation channel, SCAN channel, Transmembrane protein 16F) is encoded by the Ano6 (also known as Tmem16f) gene (Gene ID: 105722) in murine species. ANO6/TMEM16F is a multi-pass membrane glycoprotein with six cytoplasmic domains, ten transmembrane domains, and four extracellular domains. It is predominant expressed in epithelial tissues and is also found in skeletal system where it is primarily expressed in osteoblasts. It serves as a small-conductance calcium-activated nonselective cation (SCAN) channel that acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system. In osteoblasts it is essential for the deposition of hydroxyapatite during bone mineralization. ANO6/TMEM16F displays calcium-dependent phospholipid scramblase activity whereby it scrambles phosphatidylserine, phosphatidylcholine, and galactosylceramide. It is shown to be a key component of the plasma membrane repair mechanism triggered by pore-forming agents, such as bacterial toxins. This is achieved by activation of ANO6/TMEM16F by an increase in intracellular calcium resulting from flux of extracellular calcium ion. ANO6/TMEM16F is also shown to generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat cells . (Ref.: Wu, N., et al. (2020). Cell Rep. 30(4); 1129-1140).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ANO6/TMEM16F, Cat. No. ABS2210, is a rabbit polyclonal antibody that detects Anoctamin-6 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applicaitons

      Western Blotting Analysis: A representative lot detected ANO6/TMEM16F in Western Blotting applications (Wu, N., et. al. (2020). Cell Rep. 30(4):1129-1140.e5).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 65 amino acids from the C-terminal region of murine Anoctamin-6 (TMEM16F).
      EpitopeC-terminal half
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Anoctamin-6 (TMEM16F) in murine species. It targets and epitope within the C-terminal region and detects both isoforms 1 and 2.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ano6
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~58 and 110 kDa observed for isoforms 2 and 1; 55.73 and 106.26 kDa calculated for isoforms 2 and 1, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse thymus tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANO6/TMEM16F in Mouse thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      ABS2210-25UL 04065266060010