Millipore Sigma Vibrant Logo

NA02 Anti-2,2,7-Trimethylguanosine Mouse mAb (K121)

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) is validated for use in Affinity Purification, ICC, IP, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
NA02
Visualizza prezzi e disponibilità

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Prezzi e disponibilità

Numero di catalogo DisponibilitàConfezionamento Qtà/conf Prezzo Quantità
NA02-100UG
Verifica della disponibilità in corso...
—
Disponibilità limitata Disponibilità limitata
A magazzino 
Fuori produzione
Disponibili quantità limitate
La disponibilità deve essere confermata
    Prodotti rimanenti: riceverà un nostro avviso
      Prodotti rimanenti: riceverà un nostro avviso
      Will advise
      Contatti il Servizio Clienti
      Contact Customer Service

      		 Fiala di plastica 100 μg
      Ricerca del prezzo in corso...
      Non è stato possibile trovare il prezzo
      La quantità minima deve essere un multiplo di
      Maximum Quantity is
      Al termine dell'ordine Maggiori informazioni
      Lei ha salvato ()
       
      —
      Richiedi il prezzo
      		 —
      Description
      OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
      Catalogue NumberNA02
      Brand Family Calbiochem®
      Application Data
      Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
      References
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlTransfer RNA (tRNA)
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
      Key Applications Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesAffinity Purification (use Cat. No. NA02A, see application references)
      Immunocytochemistry (0.5-5 µg/ml)
      Immunoprecipitation (1-2 µg, see application references)
      Paraffin Sections (see application references)
      Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Numero di catalogo GTIN
      NA02-100UG 07790788053840

      Documentation

      Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Certificati d'Analisi

      TitoloNumero di lotto
      NA02

      Riferimenti bibliografici

      Panoramica delle referenze
      Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.

      Brochure

      Titolo
      Antibody Sourcebookﾙ Sixth Edition
      Scheda tecnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      ApplicationAffinity Purification (use Cat. No. NA02A, see application references)
      Immunocytochemistry (0.5-5 µg/ml)
      Immunoprecipitation (1-2 µg, see application references)
      Paraffin Sections (see application references)
      Application Data
      Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
      BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
      HostMouse
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Negative controlTransfer RNA (tRNA)
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.

      Prodotti e applicazioni correlate

      Related Products

      Numero di catalogo Descrizione  
      NA02A Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate Prezzi e disponibilità

      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies