Millipore Sigma Vibrant Logo

553009 PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

This PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
View Products on Sigmaaldrich.com
553009
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
553009-10T
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 10 t
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewRecognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf.
      Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number553009
      Brand Family Calbiochem®
      Application Data
      Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
      Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
      Mason, C.S. et al. 1999. EMBO J. 18, 2137.
      Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
      Moelling, K. et al. 1984. Nature 312, 558.
      Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
      Product Information
      FormLiquid
      FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
      Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      553009-10T 04055977193411

      Documentation

      PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      553009

      Références bibliographiques

      Aperçu de la référence bibliographique
      Yan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
      Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
      Mason, C.S. et al. 1999. EMBO J. 18, 2137.
      Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
      Moelling, K. et al. 1984. Nature 312, 558.
      Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application Data
      Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341.
      BackgroundThe raf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule. In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity. C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV. The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of ~74 kDa. Raf is widely expressed and plays an important role in cell proliferation and differentiation. Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus. Activation of raf itself is dependent on tyrosine (341) phosphorylation. Tyrosine phosphorylation is accomplished by Src tyrosine kinase.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
      FormLiquid
      FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
      Preservative≤0.1% sodium azide
      CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
      Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
      Mason, C.S. et al. 1999. EMBO J. 18, 2137.
      Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
      Moelling, K. et al. 1984. Nature 312, 558.
      Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.

      Produits & Applications associés

      Suggested Product

      Référence Description  
      71296 PhosphoSafe™ Extraction Reagent Prix & Disponibilité

      Produits associés classés par : Brand Facete

      PhosphoDetect™

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies