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CBA023 InnoZyme™ Cathepsin L Activity Kit, Fluorogenic

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CBA023
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Detection Methods
Fluorometric

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      Description
      OverviewA highly sensitive and selective fluorogenic assay for the detection of Cathepsin L from human and mouse samples. Cathepsin L is detected with the fluorogenic substrate Z-Phe-Arg-AMC (Exc. max: 360 nm; Emi. max: 460 nm). Interference from Cathepsin B is eliminated by the incorporation of CA 074, a specific cathepsin B inhibitor. This kit is also useful for screening cathepsin L inhibitors.
      Catalogue NumberCBA023
      Brand Family Calbiochem®
      Application Data
      Calibration curve generated using the AMC Calibration Standard included in the InnoZyme™ Cathepsin L Activity Kit. The assay was carried out as specified in the Cathepsin L Inhibitor Screening protocol.
      Materials Required but Not Delivered• Purified Cathepsin L (for inhibitor screening only)
      • Protein assay (for biological samples)
      • Precision pipettors or multi-channel precision pipettor; use only pipettors that are carefully calibrated to their target volume
      • 37°C incubator/shaker
      • Microplate reader capable of measuring fluorescence at wavelengths 360-380 nm for excitation and 440-460 nm for emission
      • Ice bucket
      References
      ReferencesBohne, S., et al. 2004. J. Pathol.203, 528.
      Goulet, B., et al. 2004. Mol. Cell 14, 207.
      Hook, V., et al. 2004. Biol. Chem. 385, 473.
      Potts, W., et al. 2004. Int. J. Exp. Pathol. 85, 85.
      Rousselet, N., et al. 2004. Cancer Res. 64, 146.
      Schedel, J., et al. 2004. Gene Ther. 11, 1040.
      Troy, A.M. et al. 2004. Eur. J. Cancer 40, 1610.
      Corella, R., and Casey, S.F. 2003. Biotech. Histochem. 78, 101.
      Ohashi, K., et al. 2003. Biochim. Biophys. Acta 1649, 30.
      Friedrich, B., et al. 1999. Eur. J. Cancer 35, 138.
      Cresswell P., 1998. Science 280, 394.
      Yasuma, T., et al. 1998. J. Med. Chem. 41, 4301.
      Kirschke, H. et al. 1982. Biochem. J. 201, 367.
      Product Information
      Detection methodFluorometric
      Form96 Tests
      Format96-well plate
      Kit containsCathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol.
      Positive controlCathepsin L
      Quality LevelMQ100
      Biological Information
      Assay range1.56-100 ng/ml
      Assay time1.5 - 2 h
      Sample TypeCell lysates, tissues, or purified cathepsin L
      Physicochemical Information
      Sensitivity1.2 ng/ml
      Product Usage Statements
      Intended useThe Calbiochem® InnoZyme™ Cathepsin L Activity Kit, Fluorogenic is suited for measuring Cathepsin L activity using purified preparations, cell lysates, and tissue extracts, and for screening Cathepsin L inhibitors. This assay does not measure Cathepsin B activity.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple storage requirements
      Storage ConditionsUpon arrival store the Cathepsin L Control at -70°C; store all other unopened components at -20°C. Upon initial use of the kit, the components may be stored for up to 3 months under the following conditions:

      Store at 4°C: Assay Buffer, Sample Buffer Diluent, 96-Well Plate, and Plate Sealers
      Store at -20°C: AMC Calibration Standard, CA 074 Inhibitor, Cathepsin L Inhibitor Substrate, and Reduction Reagent
      Store at -70°C: Cathepsin L Control: dispense into aliquots upon initial use; avoid freeze/thaw cycles
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol.
      Global Trade Item Number
      Référence GTIN
      CBA023-1KIT 04055977220889