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14-485M IKKβ Protein, active, 250 µg

Active, recombinant, full-length human IKKβ, containing N-terminal His6 tag, for use in Kinase Assays.

IKKβ Protein, active, 250 µg : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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14-485M
250 µg  Also available in 10 μg size (cat#14-485) and in bulk (cat# 14-485-K).
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      Replacement Information
      Description
      Catalogue Number14-485M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionIKKβ Protein, active, 250 µg
      OverviewRecombinant, full-length human IKKβ, containing N-terminal His6 tag
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, recombinant, full-length human IKKβ, containing N-terminal His6 tag, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
      Gene Symbol
      • IKBKB
      • IKK-B
      • IKK-beta
      • FLJ40509
      • IKKB
      • IkBKB
      • NFKBIKB
      • MGC131801
      • IKK2
      Protein TargetIKKβ
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyMisc
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).
      SIZE: 756 amino acids; 86564 Da
      SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
      PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
      SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight88kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of IKKtide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-485) and in bulk (cat# 14-485-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      14-485M 04053252733895

      Produits & Applications associés

      Alternative Packsize

      Référence Description  
      14-485 IKKβ Protein, active, 10 µg Prix & Disponibilité

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      Catégories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases