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343222 Biotin, Fluorescein Conjugate

Biotin, Fluorescein Conjugate : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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343222
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343222-1MG
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      Description
      Catalogue Number343222
      Brand Family Calbiochem®
      References
      ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203
      Product Information
      FormLyophilized orange solid
      Quality LevelMQ100
      Applications
      Key Applications Enzyme Immunoassay
      Immunohistochemistry
      Application NotesEIA (1:1,000)
      Tissue Staining (1:50 - 1:100)
      Application CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      343222-1MG 04055977195088

      Documentation

      Biotin, Fluorescein Conjugate FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Biotin, Fluorescein Conjugate Certificats d'analyse

      TitreNuméro de lot
      343222

      Références bibliographiques

      Aperçu de la référence bibliographique
      Gruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2007 RFH
      ApplicationEIA (1:1,000)
      Tissue Staining (1:50 - 1:100)
      DescriptionAvidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.
      FormLyophilized orange solid
      Recommended reaction conditionsFor tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50-1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1000.
      SolubilityDMF (5 mg/ml), DMSO (5 mg/ml), or Methanol (5 mg/ml)
      CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203