Millipore Sigma Vibrant Logo

ES007-500ML BCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble)

500 ml BCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble) for Western Blotting & IHC.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: Western Blotting, Immunohistochemistry
View Products on Sigmaaldrich.com
ES007-500ML
500 mL  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Key Applications
      WB, IHC
      Description
      Catalogue NumberES007-500ML
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble)
      OverviewAlthough nitroblue tetrazolium with indoxyl phosphate is considered an excellent substrate for alkaline phosphatase immunoblotting procedures, the availability of highly pure tetranitroblue tetrazolium (5-bromo-4-chloro-3-indoxyl phosphate/tetranitroblue tetrazolium) (TNBT) may now allow the immunologist and molecular biologist to achieve even greater sensitivity. Chemicon International has made available a refrigerator-stable, single component BCIP/TNBT solution for Western, Northern, and Southern techniques as well as immunohistochemical and in situ hybridization applications. The reaction product observed is a darker purple than that observed with NBT and may be useful for detection of multiple alkaline phosphatase probes when used in conjunction with BCIP/NBT. Methodology and reaction times are similar to the BCIP/NBT system, but may be shortened because of increased sensitivity.

      NOTE: DO NOT USE PHOSPHATE BUFFERS FOR WASHING. Inorganic phosphate is a powerful inhibitor of AP.
      References
      Product Information
      PresentationBCIP/TNBT in a proprietary buffer with enhancer.
      Color CodeBlack
      Applications
      Application500 ml BCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble) for Western Blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesPROCEDURE FOR BCIP/TNBT (ES007):

      1) After the final binding reaction with an AP labeled probe, wash tissue sections or membrane thoroughly in a buffer such as Tris/HCl or Tris-buffered saline containing 0.1% Tween 20. DO NOT USE PHOSPHATE BUFFERS. Inorganic phosphate is a powerful inhibitor of AP.

      2) After the final wash completely cover tissue sections or membrane with BCIP/TNBT Solution and incubate 5-15 minutes protected from light. Longer incubations may be necessary depending upon enzyme activity. If color develops almost immediately, the formazan deposits may flake off of the tissue sections or membrane. If this occurs, further dilution of the AP probe is necessary. A fine line of formazan deposit circumscribing the band or dot, with no deposit in the center, also suggests further dilution of the AP probes to be necessary.

      3) Stop the enzyme reaction by thoroughly washing tissue sections or membranes with deionized water.

      4) If desired, counterstain tissue sections in a 0.5% Neutral Red solution prepared in a Mol L(-1) acetate buffer. Hematoxylin solutions will not provide appropriate contrast. Membranes should be dried and stored protected from light. Little or no background staining should be observed on sections or membranes. Presence of excessive background staining indicates incomplete removal of non-bound AP from the sections or membrane. Increase the number of washes or the washing time to remedy this problem.

      Results:

      A fine dark purple precipitate will localize at sites of AP activity on tissue sections. Dark purple bands or dots will be visible at the sites of AP activity on membranes.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 12 months. Warm to room temperature prior to use. Discard if solution is turbid or purple.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ES007-500ML 04053252270598

      Documentation

      BCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      BCIP/TNBT Single Reagent, Blue/brown, Alkaline Phosphatase Substrate (insoluble) Certificats d'analyse

      TitreNuméro de lot
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE 3054695
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE 2942582
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE - 1982466 1982466
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE - 2179313 2179313
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE - 3207609 3207609
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE - 3538148 3538148
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE - 4078165 4078165
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE -2492215 2492215
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE -2521701 2521701
      BCIP/TNBT ALKALINE PHOSPHATASE SUBSTRATE -2608477 2608477
      Show more