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ABE2909-25UL Anti-phospho Histone H4 (Tyr51)

Anti-phospho Histone H4 (Tyr51) Antibody, Cat. No. ABE2909, is a highly specific rabbit polyclonal antibody that targets Histone H4 phosphorylated on Tyr51 and has been tested for use in in Immunocyto

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ABE2909-25UL
25 µL  
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      Description
      Catalogue NumberABE2909-25UL
      DescriptionAnti-phospho Histone H4 (Tyr51)
      Alternate Names
      • H4
      • H4Y51
      • H4pY51
      Background InformationHistone H4 (UniProt: P62805 ) is encoded by the HIST14A (also known as H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation, acetylation, and phosphorylation. Acetylation at Lys6, Lys9, Lys13 and Lys17 is shown to occur in coding regions of the genome but not in heterochromatin. Monomethylation and asymmetric dimethylation at Arg4 is reported to favor acetylation at Lys9 and Lys13. Histone H4 can be phosphorylated by Tie2 on Tyr51, which is shown to preferentially occur in the presence of core histones. The phosphorylated H4 (H4Y51) is recognized by the proto-oncogene ABL1. Histone H4 is also phosphorylated on Ser48 by PAK2, which is shown to increase the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Chromosomal aberrations involving histone H4 causes of B-cell non-Hodgkin lymphomas. (Ref.: Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody containing PBS without preservatives and 40% glycerol.
      Applications
      ApplicationAnti-phospho Histone H4 (Tyr51) Antibody, Cat. No. ABE2909, is a highly specific rabbit polyclonal antibody that targets Histone H4 phosphorylated on Tyr51 and has been tested for use in in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tyr51 phosphorylation of Histone H4 in the presence of active recombinant Tie2, following analysis by Western blotting.  (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).
      Immunofluorescence Analysis: A representative lot detected phospho Histone H4 (Tyr51) in HEK293 Tie2 cells treated with  Angiopoietin 1 ( ANG1) and ionizing radiation (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).
       
      Biological Information
      ImmunogenTwo linear peptides (phosphorylated on Tyr 51 and non-phosphorylated) corresponding to 11 amino acids from the internal region of human Histone H4.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Histone H4 phosphorylated on Tyrosine 51. It targets an epitope within 11 amino acids surrounding phosphotyrosine 51.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • HIST14A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight11.38 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HEK293 Tie2-myc cells treated with Angiopoietin 1 ( ANG1).
      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected phospho Histone H4 (Tyr51) in HEK293 Tie2-myc cells treated with Angiopoietin 1 ( ANG1) .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Référence GTIN
      ABE2909-25UL 04054839526961