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05-515 Anti-acetyl-Lysine Antibody, clone 4G12

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05-515
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, VrtIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-515
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Lysine Antibody, clone 4G12
      Background InformationIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
      Product Information
      FormatPurified
      Control
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.
      Quality LevelMQ100
      Applications
      ApplicationDetect acetyl-Lysine with Anti-acetyl-Lysine Antibody, clone 4G12 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent western blot analysis using 1 μg/mL monoclonal anti-GST (Catalog # 05-311).
      Biological Information
      ImmunogenMixture of chemically acetylated antigens; recognizes numerous proteins acetylated on a lysine, most prominently on histones.
      Clone4G12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAcetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Expected to cross-react with rat and mouse.
      Antibody TypeMonoclonal Antibody
      Modifications
      • Acetylation
      Purification MethodProtein G Chromatography
      Molecular WeightVaries depending upon the protein being detected.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and sodium butyrated treated HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ACETYL-LYSINE on 10 μg of sodium butyrated treated HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      05-515 04053252335709