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MABD34 Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1

Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: WRN, RECQ3, RECQL2 UniProt Number: Q14191
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MABD34
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABD34
      DescriptionAnti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1
      Alternate Names
      • Werner syndrome ATP-dependent helicase
      • DNA helicase, RecQ-like type 3
      • RecQ3
      • Exonuclease WRN
      • RecQ protein-like 2
      Background InformationThe Werner’s syndrome helicase (RecQ-like type 3; RecQ3; Exonuclease WRN) is a ubiquitously expressed nuclear protein belonging to the RecQ family. It functions as a helicase and exonuclease enzyme, and plays a crucial role in the formation of DNA replication centers. This protein is also involved in DNA repair and recombination, and may be involved in transcriptional events mediated by RNA polymerase II. It is regulated by phosphorylation and is targeted by the DNA-dependent protein kinase enzyme. Disrupted expression of the gene encoding the Werner’s syndrome helicase protein often results in the production of truncated Werner’s syndrome helicase proteins. Over 50 gene mutations may contribute to this effect—and lead to the development of Werner syndrome, a condition characterized by accelerated aging and early onset of age-related conditions such as cancer, atherosclerosis, and osteoporosis.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human WRN.
      Clone20A11.1
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
      Gene Symbol
      • WRN
      • RECQ3
      • RECQL2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.

      CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

      COFACTOR: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).

      SUBUNIT STRUCTURE: Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1.

      SUBCELLULAR LOCATION: Nucleus › nucleolus. Nucleus.

      POST-TRANSLATIONAL MODIFICATION: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.

      INVOLVEMENT IN DISEASE: Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.

      SEQUENCE SIMILARITIES: Belongs to the helicase family. RecQ subfamily. Contains 1 3'-5' exonuclease domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HRDC domain.
      Molecular Weight~162 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected Werner's syndrome helicase (WRN) in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABD34 04053252594922

      Documentation

      Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Werner's syndrome helicase (WRN) Antibody, clone 20A11.1 Certificats d'analyse

      TitreNuméro de lot
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2460445 2460445
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2467138 2467138
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 2342886 2342886
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3172611 3172611
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3299950 3299950
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3393427 3393427
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3589804 3589804
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3808794 3808794
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 3934339 3934339
      Anti-Werner's syndrome helicase (WRN), clone 20A11.1 - 4206978 4206978
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      Brochure

      Titre
      New Products: Volume 3, 2012