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ABS2235-100UG Anti-TFF2

Anti-TFF2, Cat. No. ABS2235, is a rabbit polyclonal antibody that detects TFF2 and is tested for use in Immunohistochemistry and Western Blotting.

ABS2235-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberABS2235-100UG
      DescriptionAnti-TFF2
      Alternate Names
      • Trefoil factor 2
      • Spasmolytic polypeptide
      Background InformationTrefoil factor 2 (UniProt: Q03404; also known as Spasmolytic polypeptide, TFF2) is encoded by the Tff2 (also known as Sml1, Sp) gene (Gene ID: 21785) in murine species. TFF2 is secreted into the mucus layer of the gastrointestinal tract of mammals where it stabilizes the mucin gel layer and stimulates migration of epithelial cells. It inhibits gastrointestinal motility and gastric acid secretion and possibly stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. It is also reported to stimulate epithelial restitution and restrain any associated inflammation. TFF2 levels are elevated in hypergastrinemic transgenic mice and reduced in gastrin-deficient mice. Gastrin treatment is shown to increase TFF2 promoter activity through cis-acting regions, containing CCAATA- and GC-rich enhancers. Pretreatment with gastrin/CCKB receptor antagonists is reported to abolish gastrin-dependent promoter activity. Inhibitor of PKC, MEK1, and PI-3 kinase are also shown to reduce gastrin-dependent TFF2 promoter activity. TFF2 levels are elevated in several pathological conditions, including gastric ulceration, duodenal ulceration, and Crohn s disease. In addition to its cytoprotective role, TFF2 also plays a role in the regulation of host immunity as evidenced by reduced inflammation in rat and mouse models of colitis following administration of recombinant TFF2. Higher expression of TFF2 is also observed in large tumors of the diffuse type. An increased prevalence of TFF2 expression is seen in tumors with advanced T and N stage and in patients with lymphatic and venous invasion. (Ref.: Dubeykovskaya, Z., et al. (2009). J. Biol. Chem. 284(6); 3650-3662; Tu, S., et al. (2007). Am. J. Physiol. 292; G1726-G1737; Dhar, DK., et al. (2003). Lab. Invest. 83(9); 1343-1352).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-TFF2, Cat. No. ABS2235, is a rabbit polyclonal antibody that detects TFF2 and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesTested applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TFF2 in mouse stomach tissue sections.

      Western Blotting Analysis: A representative lot detected TFF2 in Western Blotting application (Tu, S., et. al. (2007). Am J Physiol Gastrointest Liver Physiol. 292; G1726-G1737).

      Immunohistochemistry Applications: A representative lot detected TFF2 in Immunohistochemistry applications (Tu, S., et. al. (2007). Am J Physiol Gastrointest Liver Physiol. 292; G1726-G1737).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of mouse Trefoil factor 2 (TFF2).
      EpitopeC-terminal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Trefoil factor 2 (TFF2). It targets an epitope within 16 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tff2
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~17 kDa observed; 14.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse stomach tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFF2 in Mouse stomach tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABS2235-100UG 04065269802525