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MAB10765 Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6

This Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 is validated for use in WB for the detection of Red Fluorescent Protein.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: RFP UniProt Number: Q9U6Y8
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MAB10765
100 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB10765
      DescriptionAnti-Red Fluorescent Protein Antibody, clone 1DS-1A6
      Alternate Names
      • Red fluorescent protein
      Background InformationSince the development of Green fluorescent protein’s (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells.
      Product Information
      FormatAscites
      Control
      • Cos cell lysate with overexpression of RFP
      PresentationUnpurified mouse monoclonal ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 is validated for use in WB for the detection of Red Fluorescent Protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to sea anenome Red fluorescent protein.
      EpitopeUnknown
      Clone1DS-1A6
      HostMouse
      SpecificityThis antibody recognizes Red fluorescent protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • RFP
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.

      SUBUNIT STRUCTURE: Homotetramer.

      PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.

      BIOTECHNOLOGICAL USE: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

      SEQUENCE SIMILARITIES: Belongs to the GFP family.

      BIOPHYSICOCHEMICAL PROPERTIES: Absorption:

      Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm.

      Abs(max)=558 nm
      Molecular Weight~ 28 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Cos cell lysate with overexpression of RFP.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Référence GTIN
      MAB10765 04053252728082