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05-716-I-100UL Anti-Myosin Heavy Chain Antibody, clone A4.1025

Anti-Myosin Heavy Chain, clone A4.1025, Cat. No. 05-716-I, is a mouse monoclonal antibody that detects Myosin Heavy Chain and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

05-716-I-100UL
100 µL  
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      Replacement Information
      Description
      Catalogue Number05-716-I-100UL
      DescriptionAnti-Myosin Heavy Chain Antibody, clone A4.1025
      Alternate Names
      • Myosin-1
      • Myosin heavy chain 1
      • Myosin heavy chain 2x
      • MyHC-2x
      • Myosin heavy chain IIx/d
      • MyHC-IIx/d
      • Myosin heavy chain skeletal muscle adult 1
      Background InformationMyosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). The globular head of myosin heavy chain contains the actin-activated ATPase. It has been reported that during rest, the myosin head does interact with the actin filament due to the presence of tropomyosin. However, when muscle contracts, tropomyosin shifts out of the way to allow the globular head to engage the actin filaments. Myosin heavy chain is a conventional myosin with a N-terminal SH3-like domain (aa 37-82), a myosin motor domain (aa 86-782), and an actin-binding domain (aa 761-775). It has a rod like tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Myosin Heavy Chain, clone A4.1025, Cat. No. 05-716-I, is a mouse monoclonal antibody that detects Myosin Heavy Chain and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin Heavy Chain in lysates from L6 cells and human skeletal muscle.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.

      Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications (Laternser, S., et. al. (2018). SLAS Technol. 23(6):599-613).

      Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications (Jingxuan, Li., et. al. (2020). Genes (Basel). 11(3):259).

      Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications (Jingxuan, Li., et. al. (2020). Genes (Basel). 11(3):259).

      Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications (Srikuea, R., et. al. (2020). Sci Rep. 10(1):8239).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPartially purified myosin from adult human skeletal muscle.
      EpitopeUnknown
      CloneA4.1025
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone A4.1025 is a mouse monoclonal antibody that detects Myosin Heavy Chain.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Drosophila, Rabbit, Zebra Fish based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYH1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 223.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myosin Heavy Chain in C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      05-716-I-100UL 04065265324229

      Documentation

      Anti-Myosin Heavy Chain Antibody, clone A4.1025 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Myosin Heavy Chain Antibody, clone A4.1025 Certificats d'analyse

      TitreNuméro de lot
      Anti-Myosin Heavy Chain, clone A4.1025 - 3732988 3732988
      Anti-Myosin Heavy Chain, clone A4.1025 - 3746856 3746856
      Anti-Myosin Heavy Chain, clone A4.1025 - 3748851 3748851
      Anti-Myosin Heavy Chain, clone A4.1025 - 3757095 3757095
      Anti-Myosin Heavy Chain, clone A4.1025 - 3760533 3760533
      Anti-Myosin Heavy Chain, clone A4.1025 - 3770299 3770299
      Anti-Myosin Heavy Chain, clone A4.1025 - 3781073 3781073
      Anti-Myosin Heavy Chain, clone A4.1025 - 3782241 3782241
      Anti-Myosin Heavy Chain, clone A4.1025 - 3783550 3783550
      Anti-Myosin Heavy Chain, clone A4.1025 - 3784486 3784486
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