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IM1014 Anti-Matriptase/MT-SP1 Rabbit pAb

This Anti-Matriptase/MT-SP1 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Matriptase/MT-SP1.

Anti-Matriptase/MT-SP1 Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14

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IM1014
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

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IM1014-50UG
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      Description
      OverviewRecognizes the ~75 kDa matriptase protein in MCF-7 cells.
      Catalogue NumberIM1014
      Brand Family Calbiochem®
      SynonymsAnti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14
      Application Data
      Lane 1: Detection of human Matriptase/MT-SP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF-7 cells. Primary antibody: Anti-Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (1:1000). Detection: chemiluminescence. Lane 2,3: Detection of human Matripatase/MT-SP1 by immunoprecipitation (IP) followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF-7 cells. Antibody for IP (Lane 2): Anti- Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (20 μg/mg total protein). Negative control (Lane 3): Purified rabbit IgG (20 μg/mg total protein). Immunoblotting conditions: same as Lane 1.
      References
      ReferencesSantin, A.D., et al. 2003. Cancer 98, 1898.
      Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
      Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
      Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlMCF-7 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:500-1:2000)
      Immunoprecipitation (5-20 µg/mg lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human matriptase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      IM1014-50UG 04055977228175

      Documentation

      Anti-Matriptase/MT-SP1 Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Matriptase/MT-SP1 Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      IM1014

      Références bibliographiques

      Aperçu de la référence bibliographique
      Santin, A.D., et al. 2003. Cancer 98, 1898.
      Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
      Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
      Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Membrane-Type Serine Protease 1, Anti-Suppressor of Tumorgenicity 14
      ApplicationImmunoblotting (1:500-1:2000)
      Immunoprecipitation (5-20 µg/mg lysate)
      Application Data
      Lane 1: Detection of human Matriptase/MT-SP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF-7 cells. Primary antibody: Anti-Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (1:1000). Detection: chemiluminescence. Lane 2,3: Detection of human Matripatase/MT-SP1 by immunoprecipitation (IP) followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF-7 cells. Antibody for IP (Lane 2): Anti- Matriptase/MT-SP1 Rabbit pAb (Cat. No. IM1014) (20 μg/mg total protein). Negative control (Lane 3): Purified rabbit IgG (20 μg/mg total protein). Immunoblotting conditions: same as Lane 1.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~75 kDa matriptase protein.
      BackgroundMatriptase (MT-SP1, ACC. No. NP_068813), a type II membrane serine protease, may play important roles in cell migration and tumor cell metastasis. It is a mosaic protein with broad spectrum cleavage activity and two potential regulatory modules. Matriptase can convert hepatocyte growth factor/scatter factor to its active form, which can then activate c-Met tyrosine phosphorylation. Furthermore, protease-activated receptor 2 (PAR2) and enzymatically inactive single-chain uPA (pro-uPA) were identified as substrates of matriptase. Matriptase may regulate PAR2 mediated functions such as inflammatory responses, cell adhesion, and uPA-dependent tumor cell invasion and metastasis. The expression of matriptase is associated with a more invasive phenotype.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human matriptase
      IsotypeIgG
      Specieshuman
      Positive controlMCF-7 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Highly Toxic
      ReferencesSantin, A.D., et al. 2003. Cancer 98, 1898.
      Takeuchi, T., et al. 2000. J. Biol. Chem. 275, 26333.
      Lin, C.Y., et al. 1999. J. Biol. Chem. 274, 18231.
      Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 11054.