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MABS1933-25UG Anti-METTL3 Antibody, clone 29C8

Anti-METTL3, clone 29C8, Cat. No. MABS1933, is a mouse monoclonal antibody that detects METTL3 and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

MABS1933-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABS1933-25UG
      DescriptionAnti-METTL3 Antibody, clone 29C8
      Alternate Names
      • N6-adenosine-methyltransferase catalytic subunit
      • EC:2.1.1.348
      • Methyltransferase-like protein 3
      • hMETTL3
      • N6-adenosine-methyltransferase 70 kDa subunit
      • MT-A70
      Background InformationN6-adenosine-methyltransferase catalytic subunit (UniProt: Q86U44; also known as EC:2.1.1.348, Methyltransferase-like protein 3, hMETTL3, N6-adenosine-methyltransferase 70 kDa subunit, MT-A70) is encoded by the METTL3 (also known as MTA70) gene (Gene ID: 56339) in human. METTL3 heterodimerizes with METTL14 to form an antiparallel heterodimer, which constitutes an active methyltransferase that methylates adenosine residues at the N6 position of some RNAs and regulates various cellular processes, including differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, differentiation of T-cells, and primary miRNA processing. In response to UV caused DNA damage, METTL3 rapidly catalyzes the formation of m6A on poly(A) transcripts at DNA damage sites that leads to the recruitment of POLK to these sites. It is localized both in the cytoplasm and in the nucleus. It colocalizes with speckles in interphase nuclei. In response to UV irradiation, it is shown to colocalize to DNA damage sites. Its methyltransferase activity is inhibited by sumoylation. However, sumoylation does not affect its subcellular location or interaction with METTL14. Overexpression of METTL3 is reported in several human cancers and its knockdown is known to promote apoptosis. METTL3 is reported to be strongly and directly phosphorylated by ERK2 at serine 43, 50, and 525 and to a lesser extent by p38 and JNK. METTL3 phosphorylation increases its USP5-mediated deubiquitination that leads to its stabilization. Phosphorylation of METTL3 is reported to promote murine embryonic stem cell differentiation. Clone 29C8 is suitable for complex purification of both METTL3 and-14 and is useful in simultaneous analysis of both proteins. (Ref.: Sun, H-L., et al. (2020). Mol. Cell. 80(4); 633-647; Schöller, E., et al. (2018). RNA 24(4); 499-512; Wang, P., et al. (2016). Mol. Cell. 63(2); 306-317).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-METTL3, clone 29C8, Cat. No. MABS1933, is a mouse monoclonal antibody that detects METTL3 and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunoprecipitation
      Application NotesTested Applications

      Immunoprecipitation Analysis: A representative lot immunoprecipitated METTL3 in Immunoprecipitation applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

      Western Blotting Analysis: A representative lot detected METTL3 in Western Blotting applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

      ELISA Analysis: A representative lot detected METTL3 in ELISA applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 15 amino acid from the N-terminal region of human METTL3.
      EpitopeN-terminal
      Clone29C8
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 29C8 is a mouse monoclonal antibody that detects METTL3. It targets an epitope within 15 amino acids from the N-terminal region.
      IsotypeIgG2cκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Canine, Bovine, Monkey, Porcine, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • METTL3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~65 kDa observed; 64.47 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from wild-type C643 cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected METTL3 in lysate from wild-type C643 cells, but not in lysate from METTL3 knockout C643 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABS1933-25UG 04065270647153