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MAB2250Z Anti-Integrin β1 Antibody, clone 21C8, azide free

This Anti-Integrin β1 Antibody, clone 21C8, azide free is validated for use in FC, IP, FUNC, IH for the detection of Integrin β1.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: LAMB2, S-laminin, LAMS UniProt Number: P55268
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MAB2250Z
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, FUNC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2250Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone 21C8, azide free
      Alternate Names
      • CD29
      • MAB2250
      Product Information
      FormatPurified
      PresentationPurified immunoglobilin. Liquid in PBS, no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Integrin β1 Antibody, clone 21C8, azide free is validated for use in FC, IP, FUNC, IH for the detection of Integrin β1.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Affects Function
      • Immunohistochemistry
      Application NotesImmunoprecipitation

      Immunohistochemistry on frozen sections

      Flow cytometry

      Stimulates adhesion of cells to extracellular matrix proteins (Wilkins et al., 1996)

      Does not bind reduced beta1 integrin on Western blot

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogensource is Jurkat T-leukemic cell line
      Clone21C8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes distinct elements within the same epitope cluster bound by MAB2251.(Wilkins et al., 1996)
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMB2
      • S-laminin
      • LAMS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 1798 amino acids; 195981 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin).
      SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction.
      DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
      DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
      SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAliquot using sterile technique and maintain at 2-8°C.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      MAB2250Z 04053252660016