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MABN92-AF555 Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 555 Conjugate

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 555 Conjugate is an antibody against Iba1/AIF1 for use in Immunohistochemistry.

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Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: AIF1, G1, IBA1 UniProt Number: P55008
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MABN92-AF555
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIHCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMABN92-AF555
      DescriptionAnti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Allograft inflammatory factor 1
      • AIF-1
      • Daintain
      • Interferon gamma responsive transcript
      • Ionized calcium-binding adapter molecule 1
      • Protein G1
      Background InformationAllograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 555 Conjugate is an antibody against Iba1/AIF1 for use in Immunohistochemistry.
      Key Applications
      • Immunohistochemistry
      Application NotesThe unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.
      Biological Information
      ImmunogenLinear peptide corresponding to a human Iba1/AIF1 sequence.
      Clone20A12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AIF1
      • G1
      • IBA1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human spleen tissue.

      Immunohistochemistry Analysis: A 1:150 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Référence GTIN
      MABN92-AF555 04055977301205