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MABF2674-25UL Anti-IL-18 Antibody, clone 11-4.1

Anti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

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MABF2674-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberMABF2674-25UL
      DescriptionAnti-IL-18 Antibody, clone 11-4.1
      Alternate Names
      • Interleukin-18
      • Iboctadekin
      • Interferon gamma-inducing factor
      • IFN-gamma-inducing factor
      • Interleukin-1 gamma
      • IL-1 gamma
      Background InformationInterleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon  synthesis from T-helper 1 and natural killer cells. Clone 11-4.1 is shown to block IL-18 evolved interferon release in a dose-dependent manner (IC50 = 14.2 nM). (Ref.: Nariai, Y., et al. (2019). Arch. Biochem. Biophys. 663; 71-82).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Inhibition
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

      Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

      Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.

      Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length active recombinant human Interleukin-18 (without the propeptide).
      EpitopeN-terminal half
      Clone11–4.1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 11-4.1 is a mouse monoclonal antibody that detects human IL-18 protein. It targets an epitope within 6 amino acids from the N-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL18
      • IGIF
      • IL1F4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~19 kDa observed; 22.33 kDa calculated (for full-length IL-18). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2674-25UL 04065265944793