Millipore Sigma Vibrant Logo

386032 Anti-Hsp70 Mouse mAb (C92F3A-5)

Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).

Anti-Hsp70 Mouse mAb (C92F3A-5) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Heat Shock Protein 70

View Products on Sigmaaldrich.com
386032
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
386032-50UG
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 50 μg
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewRecognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
      Catalogue Number386032
      Brand Family Calbiochem®
      SynonymsAnti-Heat Shock Protein 70
      Application Data
      Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

      Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

      Substrate binding of Hsp70 in complex with a substrate peptide.
      References
      ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive controlL929 cells, Human colon cancer tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesELISA (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      ImmunogenHsp70 from HeLa cells
      ImmunogenHuman
      CloneC92F3A-5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      386032-50UG 04055977189889

      Documentation

      Anti-Hsp70 Mouse mAb (C92F3A-5) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Hsp70 Mouse mAb (C92F3A-5) Certificats d'analyse

      TitreNuméro de lot
      386032

      Références bibliographiques

      Aperçu de la référence bibliographique
      Hang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-June-2009 JSW
      SynonymsAnti-Heat Shock Protein 70
      ApplicationELISA (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application Data
      Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

      Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

      Substrate binding of Hsp70 in complex with a substrate peptide.
      DescriptionMouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
      BackgroundThe Hsp70 family consists of multiple members, all of which bind ATP in vitro, that are localized within different intracellular compartments. Hsp70 is not usually expressed under normal growth conditions, but is expressed at high levels in cells experiencing stress. It is found in the cytosol, nucleus, and nucleolus.
      HostMouse
      Immunogen speciesHuman
      ImmunogenHsp70 from HeLa cells
      CloneC92F3A-5
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlL929 cells, Human colon cancer tissue
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.