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MABF1978-25UL Anti-Complement C3a/C3a (desArg) Antibody, clone K13/16

This mouse monoclonal Anti-Complement C3a/C3a (desArg), clone K13/16 Antibody, Cat. No. MABF1978 is validated for use in Immunohistochemistry (Paraffin) Agonist or Inhibitor, Neutralizing and Flow Cytometry, for the detection of Complement C3.

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Research Category: Inflammation & Immunology Gene Symbol: C3, CPAMD1 UniProt Number: P01024
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MABF1978-25UL
25 µL  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), AI, NEUT, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1978-25UL
      DescriptionAnti-Complement C3a/C3a (desArg) Antibody, clone K13/16
      Alternate Names
      • Complement C3a
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      Background InformationComplement C3 (UniProt: P01024; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID: 718) in human. C3 is a secreted protein that plays a key role in the activation of the complement system. It s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. C3 has an anaphylatoxon-like domain (aa 693-728) and a NTR (Netrin) domain (aa 1518-1661). IC3 precursor is first processed by the removal of four Arginine residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b. C3a appears to be important in many inflammatory respons-es and the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, it acts as a chemoattractant for neutrophils. Defects in C3 gene can cause complement component 3 deficiency that leads to recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Defects in C3 gene also cause age-related macular degeneration and hemolytic uremic syndrome that can lead to hemolytic anemia and renal failure
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis mouse monoclonal Anti-Complement C3a/C3a (desArg), clone K13/16 Antibody, Cat. No. MABF1978 is validated for use in Immunohistochemistry (Paraffin) Agonist or Inhibitor, Neutralizing and Flow Cytometry, for the detection of Complement C3.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Agonist or Inhibitor
      • Neutralizing
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Complement C3a/C3a (desArg) in human liver and human tonsil tissue.

      Agonist or Inhibitor Analysis: Administration of C3a induces a transient influx of Ca2+ release in a dose dependent manner (fluo-3 staining on human PMNs). In this regard, it functions as an agonist for the C3a receptor (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).

      Neutralizing Analysis: A representative lot detected Complement C3a/C3a (desArg) in Neutralizing applications (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).

      Flow Cytometry Analysis: A representative lot detected Complement C3a/C3a (desArg) in Flow Cytometry applications (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).
      Biological Information
      ImmunogenA full length native C3a fragment purified from human serum that was activated at 37 °C f or 1 hour by treatment with 10 mg/mL zymosan.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone K13/16 recognizes complement C3a in human tissues. It targets an epitope that is present on human C3, C3a, and C3a-desArg.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • C3
      • CPAMD1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight187 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human kidney tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Complement C3a/C3a (desArg) in human kidney tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF1978-25UL 04054839476914