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MABT1555-100UG Anti-C1GalT1 Antibody, clone 5B6

MABT1555-100UG
100 μg  
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      Aperçu

      Replacement Information
      Description
      Catalogue NumberMABT1555-100UG
      DescriptionAnti-C1GalT1 Antibody, clone 5B6
      Alternate Names
      • Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
      • EC: 2.4.1.122
      • B3Gal-T8
      • Core 1 O-glycan T-synthase
      • Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1
      • 3-galactosyltransferase 1
      • Beta-1
      • 3-galactosyltransferase
      • Core 1 beta1
      • 3-galactosyltransferase 1
      • beta3-Gal-T1
      Background InformationGlycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (UniProt: Q9NS00; also known as EC:2.4.1.122, B3Gal-T8, Core 1 O-glycan T-synthase, Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1, Beta-1,3-galactosyltransferase, Core 1 beta1,3-galactosyltransferase 1, C1GalT1, Core 1 beta3-Gal-T1, C1GalT1) is encoded by the C1GALT1 gene (Gene ID: 56913) in human. C1GalT1 is a single-pass type II membrane, disulfide-linked, homodimeric protein that displays wide expression. Its expression is higher in kidney, heart, placenta, and liver tissue. C1GalT1 is a Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. C1GalT1 is shown to require a dedicated chaperone, cosmc for its proper function. It plays a key role in angiogenesis, thrombopoiesis, and kidney homeostasis development. Loss of C1galt1 is reported to promote development of aggressive form of pancreatic ductal adenocarcinomas and increased metastasis. Knockout of C1GALT1 gene has been linked to increased tumorigenicity and truncation of O-glycosylation on MUC16, which contributes to increased aggressiveness of tumors. C1GalT1 has a short cytoplasmic domain (aa 1-6), a transmembrane domain (aa 7-29), and a long lumenal domain (aa 30-363). (Ref.: Steentoft, C., et al. (2013). EMBO J. 32(10); 1478-1488; Chugh, S., et al. (2018). Gastroenterology. 155(5); 1608-1624).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-C1GalT1, clone 5B6, Cat. No. MABT1555, is a mouse monoclonal antibody that detects Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 and has been tested for use in Immunocytochemistry and Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected C1GalT1 in Immunocytochemistry applications (Steentoft, C., et. al. (2013). EMBO J. 32(10); 1478-88; Steentoft, C., et. al. (2011). Nat. Methods. 8(11); 977-82; Schjoldager, K.T., et. al. (2012). Proc. Natl. Acad. Sci USA. 109(25); 9893-8; Campos, D., et. al. (2015). Mol. Cell Proteomics. 14(6):1616-29).
      Biological Information
      ImmunogenRecombinant fragment corresponding to the Lumenal region of human C1GalT1.
      Clone5B6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5B6 is a mouse monoclonal antibody that detects human Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GalT1). It targets an epitope within the Lumenal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C1GALT1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.20 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected C1GalT1 in HepG2 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABT1555-100UG 04061841369561

      Documentation

      Anti-C1GalT1 Antibody, clone 5B6 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-C1GalT1 Antibody, clone 5B6 Certificats d'analyse

      TitreNuméro de lot
      Anti-C1GalT1, clone 5B6 - Q3382477 Q3382477

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Probing the O-glycoproteome of gastric cancer cell lines for biomarker discovery.
      Campos, D; Freitas, D; Gomes, J; Magalhães, A; Steentoft, C; Gomes, C; Vester-Christensen, MB; Ferreira, JA; Afonso, LP; Santos, LL; Pinto de Sousa, J; Mandel, U; Clausen, H; Vakhrushev, SY; Reis, CA
      Mol Cell Proteomics  14  1616-29  2015

      Afficher le résumé
      25813380 25813380
      Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology.
      Steentoft, C; Vakhrushev, SY; Joshi, HJ; Kong, Y; Vester-Christensen, MB; Schjoldager, KT; Lavrsen, K; Dabelsteen, S; Pedersen, NB; Marcos-Silva, L; Gupta, R; Bennett, EP; Mandel, U; Brunak, S; Wandall, HH; Levery, SB; Clausen, H
      EMBO J  32  1478-88  2013

      Afficher le résumé
      23584533 23584533
      Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells.
      Schjoldager, KT; Vakhrushev, SY; Kong, Y; Steentoft, C; Nudelman, AS; Pedersen, NB; Wandall, HH; Mandel, U; Bennett, EP; Levery, SB; Clausen, H
      Proc Natl Acad Sci U S A  109  9893-8  2011

      Afficher le résumé
      22566642 22566642
      Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines.
      Steentoft, C; Vakhrushev, SY; Vester-Christensen, MB; Schjoldager, KT; Kong, Y; Bennett, EP; Mandel, U; Wandall, H; Levery, SB; Clausen, H
      Nat Methods  8  977-82  2010

      Afficher le résumé
      21983924 21983924