Amicon® Pro Purification System
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Aperçu
For more detailed information on the Amicon® Pro system, visit the Amicon® Pro system webpage.
Traditional protein purification is a long process with many steps and multiple devices. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon® Ultra filter for simultaneous elution, concentration and highly efficient buffer exchange (>99%) in a single spin.
Because of the extremely flexible, modular design of the Amicon® Pro system, you can configure the perfect device for your protein preparation.
| Amicon® Pro Application | Protocol Steps | Maximum Capacity | Benefits4 |
| Purification | |||
| With buffer exchange and/or concentration | Bind Clear and Wash Elute/Concentrate +/- Buffer Exchange |
200 µL packed resin1 | Speed No sample transfer - No loss Improved yield |
| Purification only | Bind Clear and Wash Elute |
1000 µL packed resin2 | Range of sample volumes can be processed |
| Diafiltration | Buffer Exchange +/- Concentrate | variable input3 | Speed Improved activity of purified protein |
| Antibody labeling | Buffer Exchange Labeling Reaction Wash and Concentrate +/- Buffer Exchange |
variable antibody input3 | Speed No sample transfer - No loss Improved yield Read application note |
| Depletion or Enrichment | Bind Deplete/Concentrate +/- Wash/Concentrate +/- Buffer Exchange |
200 µL packed resin1 | Speed No sample transfer - No loss |
| 1 The assay capacity is dictated by the processing limitations of the Amicon® Ultra 0.5 mL device. | |||
| 2 The Bind-Wash-Elute protocol is linearly scalable (50-1000 µL). Higher resin volumes require greater centrifugation speeds and duration to ensure filtrate clearance. | |||
| 3 Capacity depends on diafiltration centrifugation protocol. For example, the antibody labeling protocol is limited to 1.5 mL reaction volumes; this is the maximum volume that the Amicon® Ultra 0.5 mL filter can process in a 15 minute spin. | |||
| 4 Benefits are relative to other current methods | |||
Features & Benefits
- High yield: No sample transfer means no sample loss
- Gentle: Novel design enables gentle, continuous flow for efficient buffer exchange
- Fast: Go from lysate to purified protein, in buffer of your choice, in just 5 spins
- Flexible: Configure the modular device to fit a range of sample prep needs
Applications
Affinity Purification, Depletion of Abundant Proteins, Protein Enrichment, Buffer Exchange, Desalting, Clean-up of Antibody Labeling Reactions
Product Performance
