Sample Preparation for Liquid Chromatography

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Obtain more information about your molecules of interest and improved resolution in the separation of complex solutions. The newest liquid chromatography technologies, such as ultra-high performance liquid chromatography (UHPLC), feature high sensitivity and throughput. To benefit from these advanced separation systems, you need higher purity samples and cleaner mobile phases. Prepare samples and mobile phases using Merck’s high efficiency filtration products for higher quality, more consistent results, increased instrument uptime and prolonged column life.

UHPLC Sample Preparation in Liquid Chromatography

UHPLC/UPLC® is a revolutionary chromatography technique using columns packed with very small (sub-2 μm) particles. This technology provides:

• Improved resolution
• Shorter chromatographic runs
• Fast method development
• 3-10 fold decrease in reagent use/disposal costs

However, the small size of the particles used to pack UHPLC columns means that very high pressures are required to operate UHPLC instruments, posing challenges in sample and mobile phase preparation. Any impurities can create backpressure buildup in the UHPLC system, causing system failure.

Protect Your Expensive System with an Inexpensive Filter.

Membrane filtration removes contaminating particles from samples, solvents and mobile phases, increasing column life, minimizing backpressure, and preventing system failure.

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Prepare your liquid chromatography samples using:

• Millex®-LCR Syringe Filter for HPLC
• All Non-Sterile Millex® Syringe Filters
• Samplicity® Filtration System

Prepare your liquid chromatography mobile phase using:

• MilliSolve™ Filtration System
• All-Glass Filter Holder (47 mm & 90 mm)
• LCR PTFE Membrane Filter Discs
• PVDF Membrane Filter Discs
• Nylon Membrane and Net Filter Discs
• High Output / Chemical Duty Vacuum Pumps