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70777 pET Expression System 28 - Novagen

pET vectors plus host strains with induction control

pET Expression System 28 - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70777
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      Description
      OverviewpET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 28
      The pET Expression System 28 contains 10 µg each of the four versions of pET-28 (pET-28a–c(+)). The pET-28a–c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB074VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70777
      Brand Family Novagen®
      References
      Product Information
      •10 µg eachpET-28a-c(+) vector DNA
      •0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
      •0.2 mlInduction control clone, glycerol stock
      Fusion tagHis•Tag, T7•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70777-3 04055977273205

      Documentation

      pET Expression System 28 - Novagen Certificates of Analysis

      TitleLot Number
      70777

      Citations

      Title
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    • Brian P. Anton, et al. (2008) RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. Procedings of the National Academy of Science 105, 1826-1831.
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    • Jennifer L. Farmer, et al. (2008) Galectin 15 (LGALS15) functions in trophectoderm migration and attachment. Federation of American Societies for Experimental Biology Journal 22, 548-560.
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    • Marie-France Langelier, et al. (2008) A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordinates DNA-dependent enzyme activation. Journal of Biological Chemistry 283, 4105-4114.
    • Lluis Masip, et al. (2008) Laboratory evolution of Escherichia coli thioredoxin for enhanced catalysis of protein oxidation in the periplasm reveals a phylogenetically conserved substrate specificity determinant. Journal of Biological Chemistry 283, 840-848.
    • Bidyottam Mittra, et al. (2008) The TbMTr1 spliced leader RNA cap 1 2 '-O-Ribose methyltransferase from Trypanosoma brucei acts with substrate specificity. Journal of Biological Chemistry 283, 3161-3172.
    • Sunish Kumar Radhakrishnan, Martin Thanbichler and Patrick H. Viollier. (2008) The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Genes and Development 22, 212-225.
    • A. Reza Radjabi, et al. (2008) Thrombin induces tumor invasion through the induction and association of matrix metalloproteinase-9 and 1-integrin on the cell surface. Journal of Biological Chemistry 283, 2822-2834.
    • Dashuang Shi, et al. (2008) The crystal structure of N-acetyl-L-glutamate synthase from Neisseria gonorrhoeae provides insights into mechanisms of catalysis and regulation. Journal of Biological Chemistry 283, 7176-7184.
    • Shalini Thakran, et al. (2008) Identification of Francisella tularensis lipoproteins that stimulate the Toll-like receptor (TLR) 2/TLR1 heterodimer. Journal of Biological Chemistry 283, 3751-3760.
    • Antonella Antignani and Richard J. Youle. (2007) The cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), can deliver Bcl-XL as an extracellular fusion protein to protect cells from apoptosis and retain differentiation induction. Journal of Biological Chemistry 282, 11246-11254.
    • Carol S. Baker, et al. (2007) CsrA inhibits translation initiation of Escherichia coli hfq by binding to a single site overlapping the Shine-Dalgarno sequence. Journal of Bacteriology 189, 5472-5481.
    • Priscilla Braglia, et al. (2007) Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae. Molecular and Cellular Biology 27, 1545-1557.
    • Nicholas E. Burgis and Richard P. Cunningham. (2007) Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase. Journal of Biological Chemistry 282, 3531-3538.
    • Elena Curti, Stephen J. Smerdon and Elaine O. Davis. (2007) Characterization of the helicase activity and substrate specificity of Mycobacterium tuberculosis UvrD. Journal of Bacteriology 189, 1542-1555.
    • Michael A. Fischbach, et al. (2007) Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Procedings of the National Academy of Science 104, 11951-11956.
    • Daphna Frenkiel-Krispin, et al. (2007) Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-Vir2 complex assembly by VirE1. Journal of Biological Chemistry 282, 3458-3464.
    • Vanessa Gonzalez-Covarrubias, et al. (2007) A functional genetic polymorphism on human carbonyl reductase 1 (CBR1) impacts on catalytic activity and NADPH binding affinity.. Drug Metabolism and Disposition 35, 973-980.
    • Changhui Guan, et al. (2007) Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota. Applied and Enviornmental Microbiology 73, 3669-3676.
    • Bei Han, et al. (2007) Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41. Applied and Enviornmental Microbiology 73, 3581-3586.
    • Marcel Hohl, et al. (2007) Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity. Nucleic Acids Research 35, 3053-3063.
    • Alexander F. Jeans, et al. (2007) A dominant mutation in Snap25 causes impaired vesicle trafficking, sensorimotor gating, and ataxia in the blind-drunk mouse. Procedings of the National Academy of Science 104, 2431-2436.
    • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
    • J. Kramer, et al. (2007) Citrate sensing by the C4-dicarboxylate/citrate sensor kinase DcuS of Escherichia coli: binding site and conversion of DcuS to a C4-dicarboxylate- or citrate-specific sensor. Journal of Bacteriology 189, 4290-4298.
    • Peter Naur, et al. (2007) Ionotropic glutamate-like receptor δ2 binds D-serine and glycine. Procedings of the National Academy of Science 104, 14116-14121.
    • Biagio Pucci, et al. (2007) Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein. Journal of Biological Chemistry 282, 12574-12582.
    • Yasuo Sakamoto, et al. (2007) Overlapping roles of the methylated DNA-binding protein MBD1 and polycomb group proteins in transcriptional repression of HOXA genes and heterochromatin foci formation. Journal of Biological Chemistry 282, 16391-16400.
    • Krishna Murari Sinha, et al. (2007) Mycobacterial UvrD1 is a ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair. Journal of Biological Chemistry 282, 15114-15125.
    • Ferdinand Steinboeck, et al. (2007) The nuclear actin-related protein of Saccharomyces cerevisiae, Arp4, directly Interacts with the histone acetyltransferase Esa1p. Journal of Biochemistry (Tokyo) 141, 661-668.
    • Keith A. Stubbs, et al. (2007) Small molecule inhibitors of a glycoside hydrolase attenuate inducible AmpC mediated β-lactam resistance. Journal of Biological Chemistry 282, 21382-21391.
    • Ken-ichiro Taoka, et al. (2007) Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement. Plant Cell 19, 1866-1884.
    • Susanne Textor, et al. (2007) MAM3 catalyzes the formation of all Aliphatic glucosinolate chain lengths in Arabidopsis. Plant Physiology 144, 60-71.
    • Giuseppe D. Tocchini-Valentini, Paolo Fruscoloni and Glauco P. Tocchini-Valentini. (2007) The dawn of dominance by the mature domain in tRNA splicing. Procedings of the National Academy of Science 104, 12300-12305.
    • Daqi Tu, et al. (2007) Structure and function of the yeast U-box-containing ubiquitin ligase Ufd2p. Procedings of the National Academy of Science 104, 15599-15606.
    • Spencer M. Whitney and Robert E. Sharwood. (2007) Linked rubisco subunits can assemble into functional oligomers without impeding catalytic performance. Journal of Biological Chemistry 282, 3809-3818.
    • Alexander T. H. Wu, et al. (2007) PAWP, a sperm specific WW-domain binding protein, promotes meiotic resumption and pronuclear developmen
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      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB074VM pET-28a-c(+) Vector Map

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