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23-023 SCF-Skp2 Protein Complex, Active, 10 µg

Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

SCF-Skp2 Protein Complex, Active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
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23-023
10 µg  
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      Overview

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E3
      Description
      Catalogue Number23-023
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionSCF-Skp2 Protein Complex, Active, 10 µg
      OverviewC-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
      Alternate Names
      • SCFSkp2
      • Skp2/Cks1 complex
      Background InformationThe SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition. Complex formation of SCFSkp2 with Cks1 is required for the degradation of p27. An increased level of Skp2 and reduced level of p27 is linked to poor prognosis in a number of cancers
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentE3
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      Catalogue Number GTIN
      23-023 04053252294686