Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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537059
Sigma-AldrichPropidium Iodide - CAS 25535-16-4 - Calbiochem
Membrane impermeable DNA intercalator.
More>>Membrane impermeable DNA intercalator. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Catalogue Number
537059
Brand Family
Calbiochem®
References
References
Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11. Pollice, A.A., et al. 1992. Cytometry13, 432.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
29-August-2008 RFH
Description
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
Form
Dark red solid
CAS number
25535-16-4
RTECS
SF7949600
Chemical formula
C₂₇H₃₄N₄I₂
Structure formula
Solubility
H₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Irritant
References
Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11. Pollice, A.A., et al. 1992. Cytometry13, 432.
