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539576 PKA, Catalytic Subunit, Bovine Heart

PKA, Catalytic Subunit, Bovine Heart MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit

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539576
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Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
539576-25UG
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      Description
      OverviewNative PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
      Note: 25 µg ≥ 500,000 units.
      Catalogue Number539576
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      Application Data
      References
      ReferencesCorbin, J.D., et al. 1988. Methods Enzymol 159, 74.
      Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
      Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
      FormLiquid
      FormulationIn 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥20,000 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      539576-25UG 04055977194739