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616382 Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem

Inhibits trypsin-like serine proteinases.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TLCK

Primary Target: trypsin like proteases
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616382
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4238-41-9C₁₄H₂₁ClN₂O₃S . HCl

Pricing & Availability

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616382-250MG
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      616382-50MG
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      Fulfillment and delivery delayed Fulfillment and delivery delayed
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      Discontinued
      Limited Quantities Available
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          Will advise
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          		 Plastic ampoule 50 mg
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          Description
          OverviewInhibits trypsin-like serine proteinases. Irreversibly inactivates trypsin without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to the target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemia cells and normal thymocytes.
          Catalogue Number616382
          Brand Family Calbiochem®
          SynonymsTLCK
          References
          ReferencesGriscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
          Kim, H., et al. 1995. J. Immunol. 154, 4741.
          Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
          Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
          Bruno, S., et al. 1992. Leukemia 6, 1113.
          Lee, S.F. 1992. Infect. Immun. 60, 4032.
          Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.
          Product Information
          CAS number4238-41-9
          ATP CompetitiveN
          FormOff-white solid
          Hill FormulaC₁₄H₂₁ClN₂O₃S . HCl
          Chemical formulaC₁₄H₂₁ClN₂O₃S . HCl
          Hygroscopic Hygroscopic
          ReversibleN
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary Targettrypsin like proteases
          Primary Target IC<sub>50</sub>EC50 = 80 µM blocking nitric oxide synthase induced by interferon-γ and lipopolysaccharides in macrophages
          Purity≥95% by elemental analysis
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXT5160000
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          616382-250MG 04055977186284
          616382-50MG 04055977186291

          Documentation

          Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          616382

          References

          Reference overview
          Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
          Kim, H., et al. 1995. J. Immunol. 154, 4741.
          Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
          Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
          Bruno, S., et al. 1992. Leukemia 6, 1113.
          Lee, S.F. 1992. Infect. Immun. 60, 4032.
          Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure

          Citations

          Title
        • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-September-2008 JSW
          SynonymsTLCK
          DescriptionInhibits trypsin-like serine proteases. Inactivates trypsin irreversibly without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Suggesting working concentration is 10-100 µM.
          FormOff-white solid
          CAS number4238-41-9
          RTECSXT5160000
          Chemical formulaC₁₄H₂₁ClN₂O₃S . HCl
          Structure formulaStructure formula
          Purity≥95% by elemental analysis
          SolubilityAqueous solutions pH <6.0 (1 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use.
          Toxicity Irritant
          ReferencesGriscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
          Kim, H., et al. 1995. J. Immunol. 154, 4741.
          Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
          Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
          Bruno, S., et al. 1992. Leukemia 6, 1113.
          Lee, S.F. 1992. Infect. Immun. 60, 4032.
          Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.
          Citation
        • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
          		•