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444235 MMP-13 Substrate, Fluorogenic - Calbiochem

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444235
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444235-1MG
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      Description
      OverviewA quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).
      Catalogue Number444235
      Brand Family Calbiochem®
      SynonymsCollagenase-3 Substrate, Fluorogenic
      References
      ReferencesKnauper, V., et al. 1996. J. Biol. Chem. 271, 1544.
      Product Information
      ATP CompetitiveN
      FormYellow lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₅₁H₆₅N₁₃O₁₅
      Chemical formulaC₅₁H₆₅N₁₃O₁₅
      ReversibleN
      Quality LevelMQ100
      Biological Information
      Primary TargetA quenched fluorescence substrate for MMP-13 (collagenase-3)
      Primary Target IC<sub>50</sub>kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceMCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Global Trade Item Number
      Catalogue Number GTIN
      444235-1MG 04055977186420