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48-621MAG MILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate s

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
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48-621MAG
96-well plate  
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      ATR (Total) Chk1 (Ser345) Chk2 (Thr68) H2A.X (Ser139) MDM2 (total) p21 (Total) p53 (Ser15) H, M, RMplexLuminex xMAP
      Description
      Catalogue Number48-621MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:

      • ATR (total)
      • Chk1 (Ser345)
      • Chk2 (Thr68)
      • H2A.X (Ser139)
      • MDM2 (total)
      • p21 (total)
      • p53 (Ser15)
      Product Information
      Detection methodLuminex xMAP
      HS Code3002 15 90
      ConfigurationPremixed
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 1 (provided).
      • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • ATR (Total)
      • Chk1 (Ser345)
      • Chk2 (Thr68)
      • H2A.X (Ser139)
      • MDM2 (total)
      • p21 (Total)
      • p53 (Ser15)
      Assay PanelEverything you need in a single kit.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Global Trade Item Number
      Catalogue Number GTIN
      48-621MAG 04053252936067